Is the quality of our RNA sufficient for RNA sequencing?

Hi, We are checking the integrity of RNA isolated (using MagMAX 96 Total RNA Isolation kit) from mice tissue on a 1% agarose gel that contains a bit of bleach to get rid of RNases. Our 28S/18S...

23 November 2020 5,006 3 View

Free database for auditory stimuli?

Hi, I'm currently looking for auditory stimuli (list of letters; word/non-words; etc) to include as distraction task in my experiment. Does anybody know a free database that I might download?...

04 October 2020 9,614 3 View

How do I give glucose solution to rats wistar after STZ intra-peritoneal dose to prevent hypoglycemic shock?

How to administer the 10% glucose dose in the rats after the STZ dose? Is the solution placed in the drinkers or is it given intragastrically? if it is intragastric, in how many ml to dissolve the...

19 July 2020 4,178 2 View

Book for demographic modelling?

Dear all, can anyone recommend a good introduction to demographic modelling in population genetics? I am mainly interested in books but paper suggestions are also welcome.Thanks a lot! Best...

10 June 2020 8,626 2 View

How education and entrepreneurship is articulated in your country?

In Ecuador, entrepreneurship is in vogue. The country has a legal framework that normalizes the articulation of Education and Entrepreneurship. In addition, Ecuador has the highest rate of Total...

15 July 2019 7,807 6 View

DGGE. Does anyone have had problems with the separation of fungal bands on acrylamide gels?

24 October 2018 8,251 6 View

DGGE. Does anyone have had problems with the separation of fungal bands on acrylamide gels?

09 October 2018 8,651 7 View

What causes a failure of read error correction in Quorum (MASURCA)?

Dear all, I am currently trying to run the MASURCA assembler with my Illumina paired-end reads. The first steps work fine but when it comes to the error correction with Quorum the program reports...

13 July 2016 7,629 2 View

Extraction from bee museum specimens?

Dear all, I aim to amplify several nuclear genes (500-1100 bp) for dried museum specimens of the bee genus Rediviva (family: Melittidae). We got one middle leg per specimen for extraction...

29 June 2016 6,792 6 View

What kind of measurement scales can I use in a questionnaire, when the statistical technique is PLS?

In my research model the independent variables have questions Likert scale , while the dependent variables are dichotomous questions; with PLS Can I combine these types of questions?

30 October 2015 3,282 2 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

Is there any expert in Smart PLS who can help me?

Dear colleagues, I have a manuscript that needs an expert in smart PLS to run the analysis, Is there anyone expert and ready to help me, please?

01 March 2021 8,212 3 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

How to run Cross-lagged panel Design on AMOS?

25 February 2021 3,126 1 View