Any suggestions for a PBS blocking buffer at pH 6.0 without albumin?

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Newton Verbisck

Hi Pia,

if non-fat dry milk, which has low albumin content, doen´t work well for you try purified casein or salmon serum.

Alejandro Martin

You might try PVP. See PMID 8095775.

Hope it helps!

Ziguo Zhang

I had similar problem sometime ago. In the end, I used non-fat milk as blocker overnight. Then wash thoroughly before incubated with antibody/detection agent. Ths Separating blocking and antibody(s) incubation solved my problem.

Geoff Margison

Be aware that non-fat dried milk contains biotin. If you are dealing with streptavidin-coated plates (?? pure guess!), the sites will be blocked. But why do you need to avoid albumin?