11 Questions 98 Answers 0 Followers
Questions related from Ziguo Zhang
Dear friends, May you let me know how can I find out the transcription start site of gene CDKN1A from any database or any public resource? Thank you. ziguo
28 July 2017 4,113 2 View
Dear all, I am trying to find some sequence information about chromosome associated RNA. Please may you let me know where should head? Thank you.
27 July 2017 8,183 1 View
Dear Friend, I am after a tethering system for protein and DNA, such as laco/laci tethering system. Recently dCRISPR-Cas9-gRNA complex seems can target DNA and bind the DNA tightly, and TALEN...
03 February 2017 6,252 0 View
Dear friend, I am trying to use yeast strain BJ2168 to over-express Gal1 promoter driven GFP. I grow the strain in SC complete media-glucose to OD 0.5. After washing the cell with PBS, I replace...
08 September 2016 9,754 2 View
Dear all, I am trying to find a marker for human cell sorting. Any information will be helpful. I prefer not use GFP if possible. Thank you. ziguo
18 April 2016 5,143 3 View
Dear all, Does anyone happen to know the copy number of Baculovirus each infected cell can produce after 2-3 days post infection in normal conditions? Thank you.
13 January 2016 9,387 1 View
Dear all, I am going to clone a set of genes which express in many common tissues, eg. brain. There are a number of companies who sell such cDNA mix. It will be helpful if you can share your...
22 December 2014 9,870 2 View
Dear All, I am trying to detect synthesised peptides with SDS-PAGE. The peptides migrate with a big difference (larger) in SDS-PAGE gel in comparison with their real molecular weight. And they...
15 December 2014 8,621 7 View
I am purifying BirA from coli expression, trying to do protein in vitro biotinylation. From the GF column, the protein is separated with two peaks, one is its dimerization form. My question is...
12 March 2014 9,975 1 View
I have a protein complex that binds to Ni-NTA Resin. If I elute with Imidazole, it needs about 150 mM of it. The 150 mM imidazole strips off some of the proteins from the complex as well,...
23 November 2012 2,764 7 View
Dear friend, I am wondering any friend has tried/is trying to use BioID methodology to screen for proximate proteins by use yeast. If so, or if you know something about yeast BioID, please may you...
01 January 1970 3,261 4 View
