8 Questions 89 Answers 0 Followers
Questions related from Ziguo Zhang
Dear friends, May you let me know how can I find out the transcription start site of gene CDKN1A from any database or any public resource? Thank you. ziguo
28 July 2017 4,057 2 View
Dear all, I am trying to find some sequence information about chromosome associated RNA. Please may you let me know where should head? Thank you.
27 July 2017 8,129 1 View
Dear friend, I am trying to use yeast strain BJ2168 to over-express Gal1 promoter driven GFP. I grow the strain in SC complete media-glucose to OD 0.5. After washing the cell with PBS, I replace...
08 September 2016 9,688 2 View
Dear all, I am trying to find a marker for human cell sorting. Any information will be helpful. I prefer not use GFP if possible. Thank you. ziguo
18 April 2016 5,089 3 View
Dear all, I am going to clone a set of genes which express in many common tissues, eg. brain. There are a number of companies who sell such cDNA mix. It will be helpful if you can share your...
22 December 2014 9,816 2 View
Dear All, I am trying to detect synthesised peptides with SDS-PAGE. The peptides migrate with a big difference (larger) in SDS-PAGE gel in comparison with their real molecular weight. And they...
15 December 2014 8,560 7 View
I am purifying BirA from coli expression, trying to do protein in vitro biotinylation. From the GF column, the protein is separated with two peaks, one is its dimerization form. My question is...
12 March 2014 9,917 1 View
Dear friend, I am wondering any friend has tried/is trying to use BioID methodology to screen for proximate proteins by use yeast. If so, or if you know something about yeast BioID, please may you...
01 January 1970 3,193 4 View
