Hi!
I'm doing pigment analysis (chlorophylls and carotenoids) of seaweed, and I have some questions about the extraction and the reference system.
I'm not sure if I should use fresh or dry material for the extraction.
I understand that giving the results based on dry weight is a more reliable reference system than fresh weight since water content can vary depending on storage, but does that mean I have to use dry material for the extraction or can/should I use fresh material and then just convert the results to dry weight? (I'm doing dry matter content analysis already so I know the dry matter and water content). Hope my question makes sense.
Thank you!
Depending on the drying method, several pigments could degrade, produce artifacts, or be extracted poorly. Therefore, I used calculations that measured the dried weight and extracted pigments from fresh samples separately, correcting for this with the average of several dried weights.
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