What type of regeneration system do you prefer in a plant tissue culture study? What are the (dis-)advantages of the systems that you have been encountered so far?
Do you mean regeneration from the roots tissue as axillary regeneartion and adventitious from stem tissue? In both case there are some portions of the inner cell layers what are capable to rapid reprogramming amd plant regeneration. However, level of etxteranl hormones what require for reprogramming will be quite different even from the same species. This is dependent from intial cell stages. Good luck!
Axillary regeneration from lateral buds, apical meristem etc. And adventitious regeneration, for example from lamina explants...
Thanks for the answer. Axillary regeneration does not require cell reprogramming because cell in axiillary buds have already (at least potential) capacity for production of the intrenal hormones (IAA through TAA1/YUCCA pathway). However, "adventives" regenerations require significant cells re-programming. In addition, one should consider facts that complex explants like stem, roots always have a proption of cells (inner layers cells continuity) with high capacity to re-programming.
As extremaly case of regeneration one can consider plant regeneation from single cells (isolated mesophyll protoplasts). In this case one should create completely new programm, include start de novo of intrenal hormones production,
Because of the reprogramming mentioned, that can lead to greater somaclonal variability. I have used it when I tried to induce mutants to increase my rate. I like the consistent size of explants and uniformity in many of the species I'm working with with axillary bud regeneration. I am going to start a study trying different media components in a series of factorials and will use plant regeneration from established meristems to bring in less variability to my study looking primarily at media components.
Hi, David, Somaclonal variation appeared only if you pass cells through long peroid of un-organized growth (callus). This is happens due to relatively high level of the ROS in the callus (un-organized cells). In the case of direct induction of axiallary buds from compotent bundle sheath cells somaclonal variation is not the case. Good luck!
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