There are plenty of papers involving phytochemical analysis for certain plant species. Moreover, target compounds isolated different plant organs produced in vitro or collected from different locations ex vitro etc.... Without testing the presence of certain compounds in plants on TLC plate prior to HPLC, how much our analysis would be reliable even our referance substances come across with such peaks! of the extract?...
Dear Buhara Yucesan,
The following link may help to you.
http://books.google.co.in/books?hl=en&lr=&id=2yvqeRtE8CwC&oi=fnd&pg=PR7&dq=phytochemical+analysis+for+certain+plant+species+by+TLC+%26+HPLC&ots=xxgkR3NiYb&sig=GhFjEi3ox_ioU2JwFfFeWOzIwFM#v=onepage&q=phytochemical%20analysis%20for%20certain%20plant%20species%20by%20TLC%20%26%20HPLC&f=false
Dear Nahire, can we use HPLC alone without qualitative analysis (TLC) of the compounds isolated from plants? If so, how much it is reliable?
Dear,
TLC is used to conforms our analyte whether it is present or not,but hplc is the tool to give you the exact percentage of your analyte present in your compound .you can do qualitative as well as quantitative analysis on HPLC.
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