Callus cultures in plant regeneration systems are sometimes useful for conservation. Moreover, they are capable of new variants. What do you think about secondary metabolite production from callus mediated regeneration systems? Is it feasible to try new variants in vitro which may provide more secondary metabolites than mother plants? I think, questions should alternatively be extended over duration of callus cultures, if the new variants are the case. "Have you ever tried/seen or read any study to check metabolite profiling of the regenerants (regenerated plantlets) derived from different callus culture periods?"
Yes U can exploit variability in secondary metabolite content in callus cultures for developing new chemvariants. Commonly referred as SOMA clonal variability.
Of cause you can exploit it. Be patient, chose products of high-value and you will have succes. Phyton Biotec in Germany have done this for more the 25 years, and have had a nice revenue. So of cause, go ahead. Also be aware that you could have additional products and also get to know more about the biosynthesis. And with cultures you can perform GMO without harming the environment.
Cells dividing at a high rate as normally occurs within callus give a chance to find variants coming from spontaneous mutations and or genetic mistakes occurring during division cycles. Of course, a consequence of this random process may be an enhancement in a metabolic pathway leading to interesting compounds or the elicitation of a new product which may be also of value for industry. Effectively, this has been a methdology largely employed in the search of new interesting phytochemical compounds in the private sector. However, it is important to realize that due to its random nature (although it is of course possible to enhance the genetic variation rate by the use of mutagenic compounds or other stressful agents) this kind of experiments are very labor-consuming and may not render the expected success at short times.
When a promising variant is found, then the cell line has to be estabilized in culture and cloned in order to avoid further variations and then to ensure that the production of the desired compound is found in the following generation of cells.
There are many strategies to produce secondary metabolites by plant cell, tissue and organ culture. In some plants, secondary metabolites accumulate entirely in specific structure such as volatile oils….etc. Thus accumulation of such products occurs only if specific structure are present in the culture. In other cases, at least some degree of differentiation in a cell culture must occur before a product can be synthesized. So, callus culture and regeneration, is very important. In other cases production requires more differentiated micro plant or organ cultures (morphological differentiation). On the other hand, callus consists of heterogeneous cells, consequently getting cell line (variant) has high yield of secondary metabolites is possible. So, production of secondary metabolites via callus need to enhancement the production through selection cell line (variant) has high yield in a certain compound(s) as well as factors affecting production and enhancement of secondary metabolites such as elicitors (biotic & abiotic), precursors, …………….etc.,
These reviews maybe help: Plant Science 161 (2001) 839-851
J Pharm Bioallied Sci. 2012 Jan- Mars; 4(1): 10-20
please anybody suggest me paper about effct of secondary metabolites production on callus pigment and how to corealted phytochemical test of callus. if have anybody ans, please must be attach reference paper.
Dear Moniika
·this review helps, on shikonin pigment
Sonia Malik , Shashi Bhushan , Madhu Sharma &Paramvir S Ahuja, (2014):Biotechnological approaches to the production of shikonins: A critical review with recent updates. Critical Reviews in Biotechnology 36(2):1-14,......................Good luck
please anybody suggest me paper about effct of secondary metabolites production on callus pigment and how to corealted phytochemical test of callus. if have anybody ans ?. PLEASE WRITE RESONE
What is the light effect of secondary metabolites production on callus pigment and morphology as well as growth?. PLEASE WRITE RESONE
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