Desalting using TCEP?

Hi all, Currently working on in-gel digestion for peptide identification, other than using ziptip or centrifugal unit to desalt sample, I heard using TCEP also works well to desalt. I wish to...

03 April 2019 8,110 4 View

Cell culture techniques?

Hi all, Just out of curiosity, is there any ways to culture cell line which goes from suspension to adherent type? I am actually culturing fish spleen cells however, it does not appear attached...

10 November 2017 9,710 4 View

Membrane tracker

Hi all, I am currently expressing a membrane protein on eukaryotic cell line, I tried to use CM-DiI for membrane labeling to verify the localization of my target protein, however, effect of CM-DiI...

06 July 2017 929 1 View

Any references on what benefits mycorrhizal fungi(AMF) obtained when colonized with plants?

10 November 2016 4,937 0 View

Low competent cell growth rate?

Hi all, i am currently using rosetta competent cell to expressing my target protein, I incubated to OD~0.4, using IPTG induction in 25C overnight, however the next day OD only ~0.5, it did not...

10 November 2016 4,869 2 View

Tracker for membrane protein?

09 October 2016 4,049 4 View

Receptor protein purification difficulties?

08 September 2016 8,247 13 View

Protein expression of a type of receptor?

Hi all, I am currently trying to express a type of cell-surface receptor(full length) using E.coli bacteria, however, i only can get small amount of protein expressed after ran SDS-PAGE gel, i...

07 August 2016 2,436 7 View

What is the differences between pET29a or b or c? Which would give best result for plasmid construction of receptor type?

I am learning to construct plasmid using pET29 but there are 3 different types, what is the differences between pET29a/pET29b/pET29c?

06 July 2016 2,211 3 View

Plasmid construction for receptor type?

04 May 2016 3,069 1 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

Best plasmid could be used as a standard curve for AAV titration?

I have been trying to have a good AAV titration for my production and I have tried different plasmids to set up as a standard curve but each one behaves differently. i am trying to use ITR for...

23 February 2021 5,167 3 View

Physical-chemical formation of stalactites/stalagmites. what promotes the formation/growth of these structures?

I know, or think I do, the basic idea behind these natural structures, as from what I know they form due to water dripping from the top leaving a salt deposit behind. But started to wonder about...

22 February 2021 1,400 4 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

How to control the variants vof covid 19 in second and third waves?

A the world find means to control covid 19 by vaccination ,new variants came to spread and make the world in fear and danger, to understand the conduct of these variants, es constantly change...

21 February 2021 3,080 6 View

How to do a surface treatment of uncoated TiO2 with polymer?

Basically, I would like to know how to treat or coat on the uncoated bare TiO2 (Ti-OH rich) surface with polymer which has anchoring groups in the polymer structure like trimethoxygroups. What is...

21 February 2021 4,657 6 View

How T cells recognise non-peptide antigens?

This might sound very basic question, but please share with me an explanation of how the T cell is activated when the antigen is not a peptide? What happens? And what are the molecules involved in...

21 February 2021 564 3 View