Can Vibrio campbellii bioluminescence be obtained when cultured on Mueller-Hinton agar?

Need to check quorum sensing activity of Vibrio campbellii.

20 January 2021 570 5 View

Is this an acceptable method for determining the minimum inhibitory concentration of plant extract using macrobroth dilution technique?

17 August 2020 3,695 27 View

Why do 293T cells starts dying after lentiviral transfection?

I have a lentiviral transfection protocol on 293T cells that has been working for years, but for the past few months, my 293T cells starts de-attaching from the plate. I finally figured out it was...

16 August 2020 6,647 3 View

Is this formula correct for measuring growth inhibitory indices for synergy index?

GIIs= ZDI in combination / total of ZDIs of the two agents in single action in order to corroborate the synergistic activity of the antibiotic in combination with the plant extracts, and synergy...

23 May 2020 9,095 5 View

What is the optimum time for agar pre-diffusion of plant extract in disk diffusion method?

27 April 2020 8,665 26 View

Is this a good method for plant extraction for antimicrobial activity?

25 April 2020 9,907 35 View

Does anti-serum vaccine works against the novel coronavirus?

The anti-serum will consist of antibodies extracted from those who already recovered from the infection.

22 March 2020 5,331 11 View

How to prepare a 50% DMSO plant extract?

How to prepare 50% DMSO from 100% DMSO? What is the solution should I use to dilute it? I have water and chloroform. The plant material is initially extracted in chloroform. Need to...

16 December 2019 5,687 8 View

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30 August 2019 610 9 View

Is there a good chance of increasing IELTS band score for writing & speaking from 5.5 to 6 after a remark?

What are the chances of increasing IELTS band score for writing & speaking by 0.5 after a remark (EOR)?

27 May 2019 4,147 11 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Is anyone familiar with the cyclic compressive fatigue testing (BS EN 12697 - 25) ?

I would like to get more information on the cyclic compressive fatigue testing (BS EN 12697 - 25) which is typically done for bituminous material in pavement design. Any one can advise on the...

28 February 2021 2,004 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Can bacterial suspension be pipetted directly onto petri dish and agar poured onto it?

Hi, recently I encountered a protocol for plating which was not the usual method I have been practicing (pour LB agar onto plate, allow it to solidify, pipette bacterial suspension onto plate,...

28 February 2021 7,292 3 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

What is the most suitable resuspension buffer for TCA/acetone precipitated protein?

I need to extract protein from fermented carbon sources for Bradford assay. Most researchers experiencing insolubility of pellet in resuspension buffer. Please assist me to select most suitable...

26 February 2021 7,129 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

EMSA troubleshooting. Protein-DNA complex not migrating, cold probe lane with multiple bands. Can anyone help?

I'm running an EMSA to show the DNA binding activity of recombinant proteins versus the Wild type. Previous assays run by my research group using a different test system show the protein-DNA...

24 February 2021 8,325 1 View

Can we recognize signs of the "volatility clustering" property of a time series in its time domain plot?

My purpose is to recognize the volatility clustering property without statistical tests such as The Lagrange Multiplier (LM) test.

24 February 2021 4,150 3 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View