Do we have to seal the lower part of the well with agar or just leave it unsealed
No need to cover the well
Dear Sony Mac
Pls. find the attached files, may help in your research, good luck
http://www.sciencedirect.com/science/article/pii/S2095177915300150
http://www.sciencedirect.com/science/article/pii/S1201971203000067
http://www.hillagric.ac.in/edu/covas/vpharma/winter%20school/lectures/31%20Antibacterial%20screening%20methods.pdf
http://www.encyclopedia.com/doc/1G2-3409800016.html
http://www.academicjournals.org/article/article1380189031_Ruiz%20et%20al.pdf
http://accounts.smccd.edu/case/antibiotics4.html
http://s3.amazonaws.com/academia.edu.documents/43994547/serial_dilution.pdf?AWSAccessKeyId=AKIAJ56TQJRTWSMTNPEA&Expires=1469698869&Signature=uCkHyYVGmH5BxBe5hLINaRi9ouQ%3D&response-content-disposition=inline%3B%20filename%3DA_Sensitive_and_Quick_Microplate_Method.pdf
http://www.scielo.br/pdf/bjm/v38n2/v38n2a34.pdf
Regards
Prof. Houda Kawas
Hi,
For well diffusion assay, after making wells just add your sample no need to do anything.
Good luck.
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