I was just wondering if one could resuspend the lentivirus pellet in whatever culture medium, instead of PBS. In this way maybe the final concentration of virus can get higher when later transducing cells?
Since you will eventually add the resuspended virus to cell culture, using the medium will prevent dilution effect.
Hi
Actually, It is our routine to concentrate supernatant containing viruses and then remaining viruses with little media would be used to transduce the target cells. It is the best if you remove the equal volume from the media of cells and use media containing viruses for trasnduction.
Happy transducting
Mostafa
Yes, in our method, media is complete and we keep media containing virus in 4 degree for a few days until collecting media containing virus is done and then use it for transduction. Freezing and thawing again of viruses decrease virus transduction efficiency.
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