I want to induce the lytic cycle of EBV in Akata and Jijoye cell lines using TPA (tetradecanoyl phorbol acetate), sodium butyrate and IgG. I was wondering how long it takes before the induction is optimal and how long the induction lasts.
Are you doing induction with the agents individually or are combining them into the induction.
Na butyrate alone induces early antigen expression generally after 25-28 hours after adding the butyrate. TPA alone induces after 48-72 hours. Combining butyrate and TPA will induce in approximately 32 hours after adding the components.
You can detect early antigens between 28-36 hours while the late antigen expression is between 36-48 hours for maximum expression.
Normally around 72 hours the cells start to dye. You can look up the old references from the 1980’s. Na butyrate and TPA induction time study papers.
Janos
Article Immunochemical Characterization of Epstein-Barr Virus-Associ...
Article Induction of the Epstein-Barr virus (EBV) cycle in latently ...
Each of the agents may need some time of its own to render the effect. This has to be tested individually, studying after what time the effects are observed and is not observed at all. This is a matter of long experimental observations
Thank you very much for your answers! I had looked for papers already and although I found some info I wanted to make sure I didn't miss any key information that has already been reported.
Thank you, Dr. Luka, I'm adding the compounds separately and now I have an idea about what I can expect!
Hi everyone,
Could I have the reference number of TPA (tetradecanoyl phorbol acetate) and which company had this product?
Can you recommend me a reference protocol for using TPA to induce EBV from p3hr1 cell lines
The original paper and protocol was published by zur hausen ( Nature. 1978 Mar 23;272(5651):373-5. Persisting oncogenic herpesvirus induced by the tumour promotor TPA. zur Hausen H, O'Neill FJ, Freese UK, Hecker E. )
If you need a protocol which we routinely use in the lab I can email you if you contact me via linkedin.
Janos
@janosluka I've requested to connect you via linkedin, and I'm waiting to send me your routinely used protocol
Zeinab
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