In the ligation process. Why does the 3′OH end attack the 5'-phosphate group after the AMP attached to the 5'-phosphate of a DNA strand?

More Sohaib Ramadan's questions See All

Student writing his own Learning Outcomes - is this the way forward?

'Students just dont want to study; they are here to waste time!' I have heard this phrase many times; so are the students solely to be blamed? We will find many students who just dont seem to...

04 February 2021 8,546 22 View

What our schooling is about? What it should be about?

Our school is trying to force down knowledge on students pretty much like shoving down pre-gained information; should it not be more about letting out the inherent talent or passion of the...

11 October 2020 7,741 8 View

Is thematic analysis an individual analysis technique for qualitative research or comes under any of the 5 approaches to Qual Research??

is thematic analysis an individual analysis technique or it falls under grounded theory (As some literature says) or it is merely a technique that can be used under any of the 5 common approaches...

30 August 2020 1,916 11 View

Can satellite spectrum be reused indoors for D2D communication?

Cognitive radios can help to share the spectrum among different networks, both satellite and terrestrial. Is it possible that we use the satellite spectrum for D2D/IoT communication in an urban...

28 August 2020 8,228 7 View

Is the heat treatment in open atmosphere an effective method for decrease carbon on surface graphitic cast iron?

The target is removing or decreasing free carbon on the external surface of gray and ductile iron.

09 August 2020 612 2 View

I want an open source FDTD for SAR signal correction due to propagation in ionosphere?

SAR signal processing software or matlab code for SAR signal processing specially for SAR signal corrections due to propagation in ionosphere

26 March 2020 5,855 3 View

I need some computer tomography files of dental jaws for its Finite element Analysis?

Actually for a project of FEA class, I need to perform FEA analysis for braces application. Please guide from where could I get the CT scans files. Because CT scan files can be converted to CAD model.

18 November 2019 8,693 1 View

Why Researchers Dissolve Sodium Azide in Phosphate Buffer (0.1M) in mutagenasis?

Really i have three questions, if i can get answers about it! 1- First One, Usually researchers used Sodium Azide with concentrations (1-5 mM or 0.01-0.1%) with long time (15-20 hour) on pH=7, or...

07 October 2019 9,045 2 View

Where i can find documented and published example of machine learning dataset for landslides problem ?

I am looking for documented and published example of machine learning data-set for landslides problem I need the data-set to be tested and published in IF journal. Example of the required paper...

01 September 2019 9,572 13 View

What to do to check gene expression if unable to extract RNA from soil and feed samples?

I need to quantify genes expression for which i need to perform qPCR but the problem I am facing is difficulty in RNA extraction from feed and soil samples. Can I use genomic DNA to get some level...

31 July 2019 7,297 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Difficulty in cloning of 1kb gene into vector?

I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...

25 February 2021 3,221 7 View

Problem with ligating 2.8 kb insert into 8.5 kb plasmid?

I am trying to clone 2 copies of the same mammalian gene into the pSF-CMV-Ub-Puro Ascl (contains HindIII, KpnI and NheI cut sites) plasmid. This is what the final product is supposed to look like...

24 February 2021 6,310 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What is the optimum time and temperature of blanching and pre-frying potato for frozen fries?

I am helping a local home industry for developing frozen fries from their local farm. I want to know what is the optimum blanching and pre-frying temperature and time for processing. Please also...

23 February 2021 1,616 5 View