Is it strictly recommended to run samples in duplicate during SNP genotyping analysis?

02 February 2021 8,877 3 View

How to present and discuss results, when methodology is not data-based?

Dear Everyone! I'd ask you opinion about a problem, which is returning in my "softer" research field. Time to time I work on research projects, which do not have available data and/or the data...

29 December 2020 7,637 6 View

How would you describe the most relevant research questions and directions in urban air pollution, if I'm specially interested in transportation?

I am relatively new to this field, my research interests are smart cities, shared mobility, transport service integration. We have data about CO, NO2, O3, PM10 from several cities. I would like to...

11 November 2020 9,099 4 View

What are the best scales to measure the role of Artificial Intelligence in trade/commerce?

I'm looking for scales to measure the role of Artificial Intelligence in trade/commerce. I'd be glad if you could recommend some valuable papers in this field too. Thanks a lot!

24 June 2020 4,209 3 View

Golgi-cox troubleshooting?

Hello there, I am having mixed results with golgi-cox staining and I was wondering if anyone has experience/insight into the individual stepwise process. My issue is that the neurons are not...

03 November 2019 2,911 2 View

Can I store my cells for few days at 4 C before FACs analysis?

I want to ask if it is possible to store my cells 24 hours post transfection at 4 C for 4-5 days (Non-fixed) just in normal media as one of my conjugated antibody shipment is delayed and will be...

10 July 2019 5,109 4 View

How to determine molecular weight of disordered peptides from diffusion NMR data?

From diffusion NMR (DOSY) experiments, diffusion coefficients are available for ~40 AA disordered peptides. Is there a method to calculate the molecular weights using these data, that is suitable...

08 July 2019 703 3 View

What is the role of the artificial intelligence (AI) in generating social innovations?

Do you know any papers discussing the the role of AI in social innovations? I'm also interested in the best practices in this field. I intend to write a literature review. Thanks for your...

01 March 2019 2,105 3 View

Are there any data available about the mechanism of loss of effectivity to immune checkpoint inhibitors?

Despite the good effectivity of the immune checkpoint inhibitors a proportion of patients will face relapse, and will be resistant to this treatment. I am intereseted if we know anything about...

03 January 2019 8,134 3 View

Best sources for analyzing Social Innovation?

What are the best sources for analyzing social innovation? I mean journal articles, papers, research reports, etc, the "must reads". Thanks for your help!

18 October 2018 8,798 1 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View