How much Volume of Chloroform to add during the Extraction and purification of Pyocyanin from Pseudomonas Aeruginosa?

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Sourabh Kundu

Add Chloroform to a total of 50% total volume and vortex vigorously for a min or half. Let the sample settle for 10 minutes.

Add chloroform to the aqueous solution and shake it vigorously until the blue pigment pass to the chloroform phase.

Ali Safaa

Sourabh Kundu Thank you very much, We will do it

Muazzam Sheriff Maqbul

Article Extraction and purification of pyocyanin: a simpler and more...

Add Chloroform to a total of 50% total volume and vortex vigorously for a min or half. Let the sample settle for 10 minutes

Muazzam Sheriff Maqbul Thank you very much for sending the Article, it will help alot