How can we assay AP endonuclease involved in DNA repair?

I am cloning endonuclease in pET vector

06 October 2019 869 1 View

How can we assay endonuclease?

I am cloning a gene whose protein product is endonuclease what enzyme assays should I do after successful purification of the protein

29 September 2019 376 7 View

My protein is Cadmium binding protein. Can I use Cadmium column instead of Nickel column for purification of protein via IMAC method?

I want to do structural analysis of the protein after purification.

19 February 2019 3,656 6 View

How to compare my fault tolerance techniques with the existing fault tolerance techniques using the throughput parameter?

I want to compare my fault tolerance techniques with the existing fault tolerance techniques using the throughput parameter..how can i compare the proposed and existing techniques like retry,...

17 March 2018 4,274 2 View

Sample size and ranking?

Hi My research have 26 factors on which I have to do survey and do ranking afterwards using spss. My target audience is muslim countries for survey. 1. Using 98% confidence interval, 3% error ,...

21 September 2016 5,514 3 View

How to get a consisten staining of DiI staining for brain slice to visualise dendritic spines in hippocampus?

Dear all, I am looking for a great experience from who has an expert skill for DiI staining. I have done this technique for almost 6 months, however my data always not consistent. "Not consistent"...

13 June 2016 3,498 3 View

What are the best methods for removing contaminating gDNA from my RNA preparations which I will be using for RT-PCR?

See above.

01 June 2014 8,483 19 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

Recalcitrant seed short-mid storage method?

Dear peer scientists, Ultradry storage is not suitable for recalcitrant seeds. However, theoretically, if we can stabilize the structure of the subcellular membrane, as well as glassify the...

25 February 2021 2,442 1 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

How can I verify my centrifugal fan design for electric motor cooling?

I have radial blade centrifugal fan design which will be mounted on exhaust side of electric traction motor. The fan will be mounted on same motor shaft inside the housing. How can I verify that...

23 February 2021 2,390 3 View

What is the stability of 2 3-dihydroxyquinoxaline in acidic water?

I'm developing a UHPLC assay, and the fluorescent product is 2,3-dihydroxyquinoxaline. The reaction to produce this requires hydrochloric acid (about 1.2 M). Will storage in this acidic...

20 February 2021 5,820 4 View

Are ZnS nanoparticles moisture sensitive?

Hello Everyone, I tried to form ZnS nanoparticles using the hot injection method and was able to form the same (confirmed by XRD). When I centrifuged, after the reaction I got a kind of white...

17 February 2021 1,709 3 View

No title

17 February 2021 4,217 3 View

How can I find the thermophysical properties of high temperature microencapsulated phase change materials (MPCM)?

Hi everyone. I am looking for a high temperature MPCM. More specifically, I need the thermo physical properties of a microencapsulated phase change material with the melting temperature between...

14 February 2021 3,606 2 View

Peptide precipitation as pre-step for acid hydrolysis for amino acid analysis?

I'm currently experiencing problems during acid hydrolysis for total amino acid analysis as my first samples contain high amounts of sugar (I get too low results back). I therefore had the idea to...

14 February 2021 6,049 3 View