Is it okay If I collect the conditioned medium and centrifuge it and take the supernatant and storage it at -80 degree?
My protein is cloned in pEt 41a vector. I have shifted it from pEt 21a to pEt 41a by designing the primers with Kpn site. So that we may remove the tag later on using thrombin. But in this way...
23 April 2022 4,947 2 View
I am currently work with antifungal study. I will be doing spore counting by using hemocytometer. I have searched some info that trypan blue can be used to differentiate live and dead cells....
19 January 2022 3,591 1 View
I am studying antifungal drug, but I only have limited drug sample. So I am planning to run the experiment in microtiter plate. Is there any manual, guidelines or reference about this?
16 January 2022 7,584 0 View
I have a fungus sample (Fusarium sp.) and I need to observe the mycelial of Fusarium sp. under SEM. Any guidelines on fungus sample preparation and examination under SEM? What is the most suitable...
10 November 2021 950 5 View
I am given a task to extract rhamnolipid from P. aeruginosa. What are the ways or precautions can be done to produce high yield of rhamnolipid?
28 October 2021 7,071 2 View
Any related studies or references about this? Common species of Fusarium found in agricultural crops Factors or how of Fusarium spp. grow / Where does the infection of Fusarium come from? Viral...
05 October 2021 2,295 11 View
You have discovered a novel species of short RNA expressed only in hepatocytes but not in other cell types. It is partly complementary to excised portion of rRNA. You hypothesize that this RNA is...
10 April 2021 1,266 1 View
I have a doubt regarding whether we adopt mapping technique in social science research, were the population list is not available?
04 March 2021 1,519 3 View
I am cloning endonuclease in pET vector
06 October 2019 985 1 View
I am cloning a gene whose protein product is endonuclease what enzyme assays should I do after successful purification of the protein
29 September 2019 493 7 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
Samuel Morse, the inventor of the Morse Code, understood that certain letters in the English language occurred more frequently than others (Gallistel and King 2010). To deal with this, Morse used...
01 August 2024 4,456 2 View
I have been harvesting monocyte-derived macrophages from buffalo and cattle fresh whole blood using the same protocol for years and getting good macrophage cultures. Below is the protocol that I...
23 July 2024 4,074 2 View
We have no access of pressure plate apparatus
22 July 2024 3,488 2 View
90% of museum objects worldwide are in storage.
21 July 2024 8,038 6 View
My study uses C57BL/6J mice and I am required to store portions of the important organs in neutral buffered formalin (NBF). Could I potentially keep those tissues in the formalin for a few weeks...
06 July 2024 8,360 2 View
What is meant by cross-contamination and what causes fruits and vegetables to become contaminated during preparation and storage?
02 July 2024 9,204 0 View
In a post-harvest handling chain (harvesting, transportation, storage), which among these do you think has the highest percentage loss why?
02 July 2024 7,649 0 View
I'm currently exploring the economic implications of solar PV curtailment versus the installation of battery energy storage systems (BESS). Specifically, I'm interested in recent publications or...
27 June 2024 6,423 2 View
Hello, I recently started working in a soil testing lab and am in charge of operating a new piece of equipment in the lab. We are using 4g soil to 40ml extraction reagent, shaking the mixture,...
26 June 2024 1,265 3 View