I was preparing small RNA libraries for an NGS. For checking the results I did a cloning and test Sanger sequencing, where I ended up with the same sequence over and over again in two different samples (human and ctenophores). Can you think of any possibility this is reasonable besides that there is no sample but just contamination? A blast doesn´t reveal any annotations, but it might be a Kozak sequence (last part is always GACAGTT). Thanks in advance!!
Could it be primer-dimer? If you have low quantities of input RNA then your primers/adapters may cause falsely high quantification. Have you checked if GACAGTT features in a primer or adapter?
Hey Fiona, thanks for the answer! I´ve already checked it and unfortunately thats not the case. But you are right, it were very low RNA quantities.
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