Hi everyone,
I need to extract Campylobacter RNA while infecting amoeba cells. Does anyone know a suitable method or kit for this? My problem is to separate the bacterial RNA from the eukaryotic RNA...i am afraid this could impair my RNAseq analyse (see what bacterial genes are being expressed while Campylobacter is infecting host cells).
Thanks,
Ana
António Maximiano Fernandes
Dear seems difficult to do this approach. There is not specific kit to to this.
My suggestion is:
First need to separate bacteria from ameba, the can be done by aplying ultrasound (bath).
- Wash infected cells several time with pbs to remove unattached bacteria.
- Then put the mixture (eppendorf) in ultrasonic bath for different time
- Use a filter that allow only bacteria to pass through
- Use it to extract rna
* The washing steps is critical
Boa sorte
0 votes
0 thanks
- Badges
- Science topic
- Similar topics
- Chemistry
- Biochemistry
- Biomolecules
- Nucleic Acids
- RNA
- Small RNA
More Ana Rebelo de Carvalho Alves Vieira's questions See All
Similar questions and discussions