I use Qiagen RNeasy Micro kit for small RNA purification. My starting material are eukaryotic cells, or material previously lysed in a different lysis buffer. Therefore, cell disruption is not a problem.
Question: With which small RNA purification procedure is higher yield to be expected: the one using the QIAzol procedure or the RLT buffer protocol?
If lysis is not a problem then RLT is the best option. otherwise, QIAzol is normally preferred in case you have lysis problem. like in case of fatty tissues.
As far as I know Qiazol is optimized to retrieve also small RNAs while the RLT is used for isolation of >200nt RNAs (in our lab at least). So if you have the choice I would go for the Qiazol, works very well in my experience.
Would you not use the miRNeasy kit in this case by the way?
Melanie, thanks for your answer, I totally agree. I used Qiazol and miRNeasy kit. So far it's the best option I found.
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