Can you please help me to understand miRdeep cut off score for novel miRNAs?

We have detected novel miRNAs in our sample where cut off score falls between 0.4 to 2.4.

04 May 2016 9,423 1 View

How to perform target prediction analysis for novel miRNAs?

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04 May 2016 2,035 1 View

How to interpret Real time PCR data?

How to calculate relative gene expression in real time PCR if Ct value of reference sample is undetermined or comes almost 37 or so.

31 December 2014 9,806 9 View

Can you suggest a list of microRNAs which are specific only to mesenchymal stem cells, irrespective of its origin?

We have already studied microRNA profiling of BM-MSCs also ESCs-MSCs and Adipo derived MSCs. But I am looking for a certain set of microRNAs which are highly specific to mesenchymal stem cells.

31 December 2013 4,461 3 View

Why is chondrogenic differentiation of MSCs done in pellet culture?

Differentiation of MSCs.

09 October 2013 7,877 18 View

What is best way to carry out microRNA profiling? Also what is the best kit to extract microRNA from primary cell lines?

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05 June 2013 9,709 4 View

Media for Umbilical cord Derived Mesenchymal stem cells

Why 95% reseachers use Low Glucose DMEM with only 2mM of glutamate (instead of 4mM what we normally use) to culture human umbilical cord derived mesenchymal stem cells? is there any specific...

31 December 2012 4,561 2 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View