I checked the quality of total RNA of tomato tissues following Trizol extraction on non-denaturing gel. I am planning to submit my samples for small RNA-Sequencing. What caught my attention is the intensity of lower observed bands that are far greater than ones corresponding to the 28s rRNA band (I expected the opposite). Another thing made me confuse is related to several bands under 28S and 18s rRNAs bands. Totally, I am wondering if my samples are ok specially regarding small RNAs.
comments are highly appreciated.
As a general checklist before sequencing I would recommend you to perform an RNA-integrity measurement using Agilent assays and/or PCR using cDNA obtained from the sample as template. This gives you a better feeling for the quality of your RNA. Beyond that, I agree with Ali, your image can not properly be interpreted.
Hi
more infromation about the gel and marker used will be helpful to draw conclusions
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