How to identify conserved miRNA if related data is not available in miRBase?

I have miRNA data of a biotrophic fungal pathogen, I would like to identify conserved miRNA by homology approach, but in miRBase the fungal miRNA data is not available. Kindly provide your...

01 February 2019 5,796 2 View

How to amplify fragment of approximately 2500 bp?

08 September 2016 4,478 7 View

What does number indicates at nodes in phylogenetic analysis?

02 March 2016 9,211 13 View

Transporter prediction based on amino acid sequence?

I have an amino acid sequence of 110 which codes for a functional transporter. Utilizing which online tool and what are the possible way by which I can confirm the structure, functioning, or any...

01 February 2016 927 3 View

Why there is difference in band position in uncut plasmid and restriction digested?

31 December 2015 5,764 5 View

Double bands after restriction digestion?

31 December 2015 9,787 5 View

Is there any need to use a single digested plasmid for a good gel image?

When we use the plasmid with insert, uncut plasmid, double digested plasmid, and ladder for gel pic, why there is a need to use single digested plasmid in making a good gel image.

11 December 2015 1,537 3 View

Can someone help with blue-white screening?

I have been doing TA cloning for quite sometime. My sample is giving me only few white colonies. I took two control, the first control is plasmid with insert, which resulted in full plate white...

07 August 2015 3,358 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to upload pdf of my articles published?

How to upload pdf of my articles published?

03 March 2021 2,183 3 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

How to add a journal?

I would like to know how to register the journal Flora Montiberica (ISSN 1138-5952 - eISSN 1988-799X) in Research Gate.

02 March 2021 8,156 2 View

Would adding books published in Lambert Publishers increase my RG impact?

There are some books published in LAP. Shall I add it to the RG?

02 March 2021 2,753 1 View

Is it possible to remove a preprint posted by a journal which is already rejected?

As we know many journals are posting preprints (DOI) in advance before the peer-review process. However, there is no guarantee that the preprints get published. The journal's reviewers undergo the...

01 March 2021 5,778 2 View

No title

Hi, I have several doubts about one paper (Systematic Literature Review) that was rejected. The main topic is Single-Board Computers in engineering education during the period 2010-2020. The...

01 March 2021 9,015 3 View