Dear Cash,
Regarding the miRNA, it depends. For example, you can try use the TRIzol method to extract total RNA (with miRNA) without any kit, but depend of which miRNA are interested for you.
In this work is discussed about the TRIzol method for extract total RNA from cells, and in what cases it is possible and which it is not. And besides that, the authors suggest some strategies that can be done to improve the extraction.
Article Short Structured RNAs with Low GC Content Are Selectively Lo...
I hope that it helps you.
Pedro
Hi Cash, i have used more than once TRIzol to extract total RNA from single mosquitoes and pools of insects (notice that it is not very easy to extract nucleic acids from mosquitoes). We used this RNA for small-RNA sequencing, focusing on Piwi-RNA, and we always had good results.
Honestly, i find TRIzol better than most kits for RNA extraction.
Thank u unberto for nice reply. it was nice to know your experience, yes it is true . But my question is regarding post sequencing validation of small RNA , what r the methods available for validating your RNA seq data for small RNA ? By the way which u hv used for library prep and what was the size of fragments in enriched library ?
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