For gelatin in SDS PAGE analysis...i got smeard band why?

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Pierre Béguin

Gelatin is a heterogeneous mixture arising from the partial hydrolysis of collagen. You can’t expect anything but a smear if you run it on an SDS-PAGE gel.

Andrew Kirkham

I was going to say the same Pierre

Tomasz Fraczyk

Probably you have a high ionic strength of the gelatin sample. It destroys the separation.