I need to target PV interneurons in 1 and 2 months old mice.
Hi there,
This paper contains interesting information as well as hippocampal CA1 coordinates
https://www.nature.com/articles/nn.2751?error=cookies_not_supported&code=d3ff7bba-b4b4-417d-84a5-fab0697c3407
Dear Rashmit Kaur
It might be useful to check a mouse brain atlas as well,
The Allen Reference Atlas (available for P56 and P14 mice):
https://mouse.brain-map.org/static/atlas
An interactive tool for stereotaxic coordinates is also available:
http://labs.gaidi.ca/mouse-brain-atlas/
Good Luck
Help me to draw graph of Bioconcentration
10 June 2024 4,941 2 View
I want to estimate total sugars in carrot and products developed from it. I have been using lane and eynon method. I could get the values for reducing sugars but not for total sugars. What is the...
28 May 2024 9,382 4 View
My name is kiranbir kaur and m student of MSC Computer Engineering in Solent University Southhampton (United Kingdom).Please suggest me the topic for dissertation.Include the Problem and Solution.
24 May 2024 4,355 2 View
Hi, My name is kiranbir kaur and m student of MSC Computer Engineering in Solent University Southhampton (United Kingdom).Please suggest me the topic for dissertation.Include the Problem and Solution.
24 May 2024 1,180 3 View
Dear researchers, I am treating the waste water by vermifiltration process using different filter bed media. After the treatment, BOD, COD, TSS were found to be reduced but reduction in total...
18 May 2024 1,897 3 View
Using 10 ml of 1% methanolic hcl, 1 g fresh sample and OD at 530 and 657nm
07 May 2024 4,440 1 View
when i am installing cooja 3.0 in ubuntu 18.04 i am facing some problem give me some solution
01 April 2024 4,305 0 View
While synthesising nanocomposite material by blending one component over another, it was observed that if amount of components is doubled/increased without increasing blending time (i.e., kept...
19 March 2024 2,781 2 View
I have knocked down my gene of interest by lentiviral transduction. I revived the vials and continue growing cells for 72 h without any selection. After confluency, I passaged the cells later 24...
28 February 2024 4,032 1 View
To calculate ethanol content, we use the formula ABV=(OG−FG)∗131.25 what is this 131.25 constant and how we can calculate it?
19 February 2024 9,034 0 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
I need to Postfix mice brains. I slice the brains using microtome at 40 microns. These are Postnatal days 7, 11, 14, and 21. I am using half the brain for other analysis, so I need to fresh freeze...
31 July 2024 1,123 4 View
We have encountered an issue with the serum of mice being hemolyzed after the process of centrifugation. Could you please provide guidance to prevent hemolysis ?
29 July 2024 8,402 4 View
I am running a western on HIV envelope protein. The size is around 50Kda and I am using rat anti-HA for the first stain and a second stain of mouse anti-CA. Primary antibody dilution is 1: 1000...
29 July 2024 6,484 5 View