10 September 2021 2 497 Report

I need to target PV interneurons in 1 and 2 months old mice.

More Rashmit Kaur's questions See All
First time secondary research, some points I should keep in mind?

I am conducting secondary research for my dissertation for undergraduation (Psychology) , what should I make sure my proposal consists of? Are there any tips I need to keep in mind?

22 February 2021 6,035 1 View

I have faced a problem to amplify VP2 gene of porcine parvovirus 1,2,3 and 5? Please suggest me how can i solve this problem?

Till now i haven't got any PCR amplified vp2 gene bands on specific annealing temperature and in step up PCR.

08 February 2021 819 4 View

Can anyone provide me with the scoring method and the questionnaire of Work life balance scale by Hayman (2005)?

Greeting! For my MA dissertation, I am thinking of taking Work life balance scale by Hayman. However, I can't find the scoring procedure; also confused about the actual rating taken. If anyone...

23 January 2021 4,074 7 View

Quantitative Research Involves 3 Different Likert Scales, how do I go about data analysis?

This is our first research paper and it is titled "Effect of Peer Connection on Attitude Towards School and Academic Motivation in Higher School Students During Pandemic". We aim to investigate...

30 December 2020 5,450 4 View

My research involves 3 diff scales about peer connect, attitude towards school and academic motivation, how should I go about finding the correlation?

This is the first time I'm conducting research and I'm unsure about the result and scoring process, kindly help

27 December 2020 1,260 3 View

Hi everyone, I am digesting my plasmid with SgrAI enzyme but it showed staractivity everytime?

My plasmid (pJET1.2) contains an insert of around 2969bp. The total size of plasmid 5942 bp. I am cutting it with AhdI, SgrAI and BssHII. Desired bands are of 2969, 1632 and 1432bp. I want to...

15 November 2020 4,761 4 View

Perceived Academic Stress?

Hello, Does anyone have any idea of how this is scored? What would be the average scores? Would would be seen as very low/high? BEDEWY D & GABRIEL A. Examining perceptions of academic...

09 November 2020 2,040 1 View

Does Shannon index represent largest diversity index when the individual belongs to different species and vice versa?

The shannon wiener index and Simpson indices include the measurement of community heterogeneity. Shannon wiener index bapplied information theory. The main measuring object of information theory...

22 October 2020 1,053 7 View

Parameters for magnesium acetate in AMBER MD simulation?

I am trying to use Magnesium acetate [Mg(CH3COO)2] as the salt instead of Sodium chloride [NaCl] in my tleap. Are there any AMBER parameters for acetate ion that I can use?

12 October 2020 931 3 View

Can anyone tell the fast publishing SCI journals?

I want to know the SCI journals of Physics - Computational Physics, Soft Matter Physics, Condensed Matter Physics which provide a fast review process.

12 October 2020 8,911 2 View

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Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

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Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does PHA-P stimulate the proliferation of lymphocytes from mice?

Hello, I will start performing MLR using lymphocytes from mice's spleen and I would like to know the better way for the stimulation. If is using PHA-P or CD3/CD28? I would like to know as well if...

23 February 2021 5,476 4 View

How to culture mouse pheochromocytoma cells (MPC)?

Hi Everyone, Has anyone here worked with MPC cell line? Any tips or tricks? I am just venturing into cell biology and any advice on this topic would be highly appreciated. Thank you so much. Niusha

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