How to identify and retrieve many (+30) RNASeq datasets (Fasta) under a specific criteria in a practical way?

22 March 2020 3,649 3 View

What have been the contributions of cultural studies to the teaching of English as a second or foreign language?

the idea that intercultural competence develops communicative competences. Worldwide, this relation intercultural-communicative competence has been studied by teachers and researchers (Agudelo,...

01 February 2020 9,056 3 View

How to conduct a Chi-square analysis dealing with two independent groups?

Hello fellow scholars, I would like assistance with a Chi-square analysis dealing with two independent groups. In one research article I was reading, the researcher was studying US attitudes...

25 January 2020 7,039 6 View

How is the Linguistic Colonialism evidenced in the English Textbooks of Multinational Publishing houses in teaching of English as a second language?

The purpose of this question is to examine and compare the concepts of linguistic colonialism and cultural alienation in University textbooks for teaching English as opposed to the theories about...

01 January 2020 1,603 3 View

How to connect beam188 and link180 elements in ANSYS APDL?

I am currently working on the design of a deployable truss structure and I would like to know what is the proper way to connect beam188 and link180 elements in APDL? The diagonals (purple lines)...

07 April 2019 3,152 4 View

What's the amplified gene in the DNA ladder assay for apoptosis detection?

Greettings, I need some guidance. Regarding to the DNA ladder assay for apoptosis detection which is based on electrophoresis analysis, I would like to know what is the gene to be amplified in...

03 April 2019 9,559 4 View

Protein misfolding behaviour?

Hi all, I am working on a tricky protein. I surpassed some initial aggregation behaviour by adding 0.3% CHAPS and 1mM TCEP, buffer in which is happy until the removal of the SUMO tag, then it...

09 December 2018 9,460 3 View

In a Real Time PCR, is a CT of 4.6 (average) enough to determine presence of babesia/anaplasma in a sample?

We are currently trying to identify presence of babesia bovis, babesia bigemina, and anaplasma marginale in DNA isolated from white blood cells. However, the amplification plot values start...

12 November 2018 4,051 3 View

Does anyone know a clustering algorithm/software that transforms a group of images into an undirected weighted graph?

Hi everyone. I'm currently trying to assess the ability of human volunteers to cluster a set of images into a fixed number of clusters according to perceived visual similarity (the images are...

14 January 2018 8,222 4 View

I need help with teaching-learning strategies in mathematics, specifically applying gamification.

I am a teacher of the differential calculus class for first semester engineering students, I would like to motivate them, so I would like some online tools or group dynamics to see some topics...

07 August 2017 9,273 8 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View