Dear colleagues,
I work in a private company, which is serving labs. One of our customer has a serious problem with contamination in the real-time pcr. First contamination was detected in February in all negative controls and negative patients (total contamination). All lab was carefully cleaned with bleach more than ones. All pipettes were opened and were placed under UV. All these mentioned facts didn't
help. Also pcr room was washed including walls, all surface and laminar boxes. Unfortunately, contamination still exist, however in the lower concentration. And we cannot find where it comes from.
Kindly ask you to comment this issue, your expert view very important for us.
P.S. Lab has been existing for 10 years, pcr has the same staff.
Before this issue they have never met such kind of problems.
DNA tests without contamination.
Could you...provide more detail?
It is essentially impossible for RNA to contaminate PCR-based DNA tests: RNA is highly labile, and moreover isn't a viable substrate for DNA polymerases.
What sort of contamination do you think it is, where do you think it comes from, and what kind of test (specifically) are you doing?
I also doubt that your contamination is from the RNA. RNA molecules are quickly degraded and RNase enzymes are literally everywhere. A much more likely answer is that your contamination is from amplified PCR products from your cDNA. Very hard to get rid of. Throw away ALL of your reagents (buffers, enzymes, water, etc.).
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