Does anyone know how to change the rotors of the Rotor-Gene 3000 Real-Time PCR machine?

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What is the best method for total RNA extraction from mice spinal cord ?

Hello, I would like to extract total RNA from mice spinal cord. Does anyone know a method or kit for this?, Thank you!

24 January 2019 2,712 2 View

Does anyone have an excel template for the analysis of single and multiple reference genes (2) by using the Pfaff method?

Hello, I need to analyze my Rea Time PCR data with the Pfaff method by using single or multiple reference genes (2). I would really appreciate if some can help me with an excel template. Thanks...

23 January 2019 7,759 3 View

Does anyone have an excel template for the analysis of single and multiple reference genes (2) by using the Pfaff method?

Hello, I need to analyze my Rea Time PCR data with the Pfaff method by using single or multiple reference genes (2). I would really appreciate if some can help me with an excel template. Thanks...

31 December 2018 1,676 3 View

What is the best method for total RNA extraction from mice spinal cord ?

Hello, I would like to extract total RNA from mice spinal cord. Does anyone know a method or kit for this?, Thank you!

31 December 2018 3,756 4 View

Can I run an ethidium bromide gel with SYBR green Real Time PCR products?

I want to see the products of my Real Time PCR (supermix prepared with SYBR Green I) by using an ethidium bromide gel. Have anyone done this?

04 May 2018 5,463 4 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Arena simulation - how do i model the following model in ARENA (see description) (new to ARENA)?

Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...

02 March 2021 2,431 3 View

Which is the most convenient manufacturing software for Additive Manufacturing?

Which tools can be used to generate machine codes for additive manufacturing?

02 March 2021 7,220 2 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View