To dear Researchers,

I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8 and it was consistence with that in Scatter plot equation. I also drew a line plot. In the line plot the slope was -3.2 and the efficiency was 100%. Also, there was no linearity in Scatter plot but there was a well fitted linearity (0.99) in line plot. I'm not sure what is the problem. Please help me figure it out.

Kind regards

Alireza

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