How to fix unstuck gel with backing during the deep purple staining of 2-D gel for protein spot picking?

03 March 2016 1 3K Report

During a process of deep purple staining of 2-D protein gel for automated Ettan DIGE spot picker, my gel was unstuck with the backing (Plate). I can’t scan the gel as it is not fix with backing. Can anyone suggest me the possible solution to fix this problem?

Johan Renes

Dear Muhammad, try bind silane from GE and carefully folly the procedure by the manufacturer. In our hands that worked well.

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