Is there any method to prepare Prion protein?

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How do i get an account to upload my published papers?

need to open an account to upload my published papers

01 August 2024 9,255 1 View

How to transfect circular mRNA to T cells?

Hello! We are using LNP to deliver circular mRNA into T cells. However, compared with linear mRNA, the transfection efficiency and protein expression duration are similar. Are LNP generation or...

21 July 2024 4,322 1 View

How do you determine the Ki of an inhibitor for an enzyme which also undergoes substrate inhibition?

There are three factors involved in the activity, the substrate acting as a substrate, the substrate acting as an inhibitor and another inhibitor for which you want to measure the Ki.

08 July 2024 4,988 1 View

Can anyone help with a sample of Kraft pulp process lime mud (CaCO3)?

Can anyone help with a sample of Kraft pulp process lime mud (CaCO3) I need a sample of 250g to run R&D lab testing. I am based in the UK and I am struggling to get hold of some lime mud...

01 July 2024 9,217 0 View

Can research gate tell me how many times a particular author has been cited?

I'm writing a letter of recommendation for a colleague and I'd like to mention how often her publications are cited by other academics.

28 June 2024 4,995 0 View

How can indigenous knowledge systems assist in mathematics problem solving?

Recent research papers and journals on indigenous knowledge system

21 June 2024 6,358 1 View

L need an assistance on how to carry out a systematic review of causes and preventive measures against maternal mortality in Ogun State, 2018-23?

l need an assistance on how to carry out a systematic review of causes and preventive measures against maternal mortality in Ogun State, Nigeria, 2018-23?

19 May 2024 9,103 1 View

Do imbedded Gaussian basis sets use core potentials (ECP)?

We are using gaussian G-16 to calculate molecular properties. When I specigy in the rout line def2qzvpp ( #P B3LYP/def2qzvpp opt output=pickett) with RU (Ruthenium atom in the atom list) --...

10 May 2024 4,810 5 View

Can Metabolic Disorders cure by medicine,sustitutes,gens,and stemcell excercises etc?

Metabolic disorders occur due to our proper habits of eating process and remineralisation in the mouth. Like food, water, and liquids, etc. Due to mutations in all our food in saliva, the...

22 April 2024 1,959 3 View

Have bears historically preyed on Atlantic salmon (Salmo salar)?

We have searched literature in Norway and North America trying to find evidence that brown or black bears preyed on Atlantic salmon (Salmo salar), despite the fact that these fish spawn in...

17 April 2024 2,262 5 View

Why does my protein refolded to beta sheet during thermal denaturation analysis?

Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...

06 August 2024 1,989 3 View

How prion can cause disease?

how is prion can cause disease

01 August 2024 250 1 View

Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

How to retain the a GFP tagged gene expression in stable cell line?

Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....

29 July 2024 5,007 0 View

What is the relationship between protein structure and N or C terminal tagging choosing?

I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...

28 July 2024 366 3 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

EDC carbodiimide and NHS easter 's half life among different pH?

It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...

25 July 2024 8,738 0 View

Does post-translational protein modification cause devisions on observed pI verses calculated pI?

In running two-dimensional gel electrophoresis on bacterial protein, some spots that appear to match a protein sequence have a significantly more acidic isoelectric point than the calculated pI....

24 July 2024 8,076 3 View

What is the function of fusion tag GAMGSGIQRPTST?

I found that several expression constructs of published protein structures containing an N-teiminal fusion tag GAMGSGIQRPTST. What's the founction of this fusion tag?

23 July 2024 1,353 4 View

The best source for amplification of ADAM17 prodomain?

hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...

21 July 2024 8,660 1 View

Fred Romano

Dr Zabel Laboratory, more focused on CWD, but a prion is a prion, in whatever animal http://csu-cvmbs.colostate.edu/academics/mip/prion-research-center/Pages/Zabel-Laboratory.aspx

A complete review of prion by the researcher that discovered it in 1976, Nobel Prize in 1996 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC33918/

Yingjun Liu

It is not easy. But it was achieved recently. Check this: https://www.nature.com/articles/s41467-018-04584-z