Hello, please i would like to know if I can add RNAlater to non-anticoagulated blood sample in order to stabilize and protect RNA. If yes, which volume to add ?
I carried out low-cycle fatigue tests on a secondary-hardenning ultra-high-strength steel with test condition εmax = 0.9%. It was found that the fatigue life at high temperature was much higher...
29 May 2024 3,176 1 View
How do researchers avoid over-amplification of eukaryotic chloroplast 16S rDNA when classifying prokaryotic microbial communities in algal bloom experiments? For example, if I need to amplify...
07 April 2024 3,064 0 View
TVET graduates perceive the incorporation of technology into their educational programs. It delves into their attitudes towards technology and its role in their vocational training.
06 March 2024 5,116 1 View
Dear community: I would like to seek opinions from you on current-clamp mode in electrophysiology. I am trying to record voltage response from neurons, so I first do whole-cell recordings on...
24 January 2024 9,549 4 View
Hi, I'd like to stain 10M cells with CD3 for cell sorting. The datasheet says 5ul/test, and typically use 1 × 10^6 cells in a 100-µl experimental sample (a test). So does it mean I need a 50ul...
26 October 2023 5,300 2 View
topic: the concrete material model RHT in LS-DYNA I see some scholars mentioned the damage value threshold Dcr, but I don not know where to set it, and find no relative paramaters in 072MAT_RHT....
06 August 2023 1,660 1 View
As below: INFO: Using cached SIF image INFO: MPS server daemon started INFO: Running QE with: 2 GPUs 4 MPI processes total 2 MPI processes per GPU 2 Pools 2 KPoints + mpirun -n 4 ph.x...
20 June 2023 187 0 View
Could anyone provide some practical LS-DYNA models for learning?
09 June 2023 6,846 1 View
Hello Researchers I would like to perform the 2D simulation of a PVT system with fins using FORTRAN. I therefore ask for help for anyone who has it or who knows a website where I can find it. The...
30 April 2023 357 2 View
i found the content of dry weight of euglena gracilis under mixtrophic is much higher than than under heterotrophic. almost 2-fold. is that right?or something wrong happened?
23 April 2023 1,902 1 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)
05 August 2024 9,537 2 View
Are there any statistical methods to justify your sampling technique using SPSS or AMOS?
05 August 2024 9,153 4 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
I am conducting a qualitative study that uses interviews to investigate the perceptions of teachers about a particular leadership practice and I am focusing on 3 schools which have a total number...
01 August 2024 8,457 10 View
I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...
31 July 2024 2,150 0 View
Hi all, My lab has Thermo Scientific™ Invitrogen™ EVOS™ FL Auto 2 Imaging System, and I was wondering if I will be able to use it with whole blood, whilst focusing on platelets? The idea would...
24 July 2024 9,337 3 View
Hello, good morning. I would like to know which plate I can use to infect fresh PBMC with virus and culture for 24 hours. I have tried using 12-well and 24-well plates, but many cells adhere and...
24 July 2024 2,158 2 View
I isolated microbial DNA from marine water samples taken at various sites using the Qiagen Power Water DNA Isolation Kit, including a modified enzymatic lysis step. I encountered a situation where...
24 July 2024 9,688 0 View
hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...
21 July 2024 8,660 1 View