Hello Muhammad Junaid Khawaja,
Blood clotting is a very complex process and it involves a number of factors, and calcium is considered as one of the most important player in the clotting process.
EDTA is a chelating agent that can bind to diavalent cations such as Ca2+. So, in the presence of EDTA, calcium is removed from the blood sample as a result of which the specific steps leading to blood clotting will not be completed, and the blood will not clot.
Best.
Simply, EDTA can remove the calcium ions from the blood ample and then blood not able to clotting. This is the good way to keep the blood in the fluid status for lab investigation purposes.
Simply, EDTA can remove the calcium ions from the blood ample and then blood not able to clotting. This is the good way to keep the blood in the fluid status for lab investigation purposes.
Hi Muhammad,
It’s important to add EDTA to a sample of blood to be the blood in a fluid condition without clotting (as the coagulation process is so complex), thus it’s easy to some special hematological tests, as well as, we can get the plasma part of the blood which contains the most important clotting proteins called ‘’Fibrinogen’’
Best Wishes
I’ll add some information to my colleagues' answers, all perfect. In contrast to other chelating agents that remove calcium (Na citrate, for example) or other anticoagulants that act with different mechanisms (heparin), EDTA better preserves cell morphology and structure. It is, therefore, the anticoagulant of choice for the WBC, RBC, and PLT counts and for their morphological evaluation in light microscopy.
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