How to induce diabetes in swiss mice (fructose)?

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What topic or subject does Production Engineering need to address more?

Contemporary scores or innovations in scientific approaches. Hybrid methodologies, emerging themes and cross-cutting issues?

27 July 2024 648 3 View

Can I use candle-jar technique to lactic acid bacteria?

I don't have the anaerobic generators (Anaeropac) so I'd like to try the candle-jar tecnique for the cultive of lactic acid bacteria

15 July 2024 8,890 2 View

Help to isolate Ralstonia from plant tissue?

I need tips for isolating Ralstonia solanacearum from eucalyptus. I have used some methods, such as isolating from root exudates, but it didn't work. I also tried indirect isolation from macerated...

02 July 2024 9,807 3 View

How do I get good bands after PCR of gDNA from bacterial cells?

I have 6 bacterial isolates, I have checked their band on agarose gel electrophoresis, I am getting a genomic band but when I am performing the PCR and after running the gel, I am not getting good...

21 May 2024 8,614 4 View

Inconsistente results with cell line LX-2, any advice?

Hi there! I'm working on my master's thesis and for my project I'm testing a new curcumin derivative in liver fibrosis. I'm working with the cell line LX-2 and have been having problems since the...

21 May 2024 2,171 0 View

How to retain the structure after optimization using Gaussian 16?

Dear Experts I have optimized an organic molecule using DFT/B3lYP/ 6-311G using Gaussian16. However, after the optimization, it was found that some bonds were missing from the molecule's...

17 May 2024 9,828 1 View

I'm having trouble extracting total RNA from a culture of macrophages derived from monocytes infected with Leishmania infantum. Can someone help me?

I start my primary cell culture in a 24-well plate with 2x10⁶/mL PBMCs obtained through Ficoll gradient. After 24 hours, I wash the wells with saline to leave only adherent monocytes (~1x10⁵) in...

12 May 2024 1,045 4 View

How can I set the distance between peptide and bilayer using Packmol?

I still find Packmol very tricky with parameters. I have two pdb files, one with a pre-quilibrated bilayer and one with the peptide of interest. How can I create a system with three copies of the...

29 April 2024 8,231 1 View

What requirements (or qualities) are most important to be a good university professor?

22 April 2024 3,009 5 View

How to calculate the solute concentration in molecular dynamics simulation?

I am trying to calculate how many SDS molecules I need in a MD box to achieve a specific concentration (ex: 1M). My first doubt is if I use the box volume or the quantity of water molecules, for...

16 April 2024 2,051 2 View

• What the possible Persistent Organic Pollutants and Heavy metals present in fluorspar, sediments, and water bodies around its mining area?

Approximate concentrations are require in compared with the WHO permissible limts

11 August 2024 2,723 1 View

Handling Missing Data and Building a Predictive Model with Incomplete Information ?

I am developing a predictive model for a water supply network that involves 20 influencing points. However, I only have historical data for 10 out of these 20 points. I would like to know how to...

10 August 2024 4,005 2 View

Do interactions between biosphere, carbon cycle, & water cycle impact global warming & interaction between atmosphere & hydrosphere?

How do interactions between the biosphere, the carbon cycle, and the water cycle impact global warming and interaction between the atmosphere and the hydrosphere?

09 August 2024 3,291 2 View

Is it true that $\det(V(A))$ may be only $\pm 1$, depending on $n$, for the last symmetric tridiagonal matrix $A$?

One can try to generalize the Vandermonde determinant in the following direction: Let $A$ be any symmetric $n$-order square matrix. Consider its powers' diagonal elements $(A^k)_{ii}$ and...

08 August 2024 6,690 1 View

Can formaldehyd-fixed gelatin be melt?

I am puzzled about the properties of gelatin, when used for tissue-embedding. Hard gelatin can be melt again by temperatures about 40°C. Formalin-fixed gelatin is like crosslinked protein and...

07 August 2024 1,686 1 View

What is saturation solubility of insulin in saliva?? I need it to know sink condition ?

05 August 2024 4,407 0 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Why am I getting "shark finn"-like signal?

My collegue and I are having hard time to understand what is happening. Basically we have a tapping device that work with Arduino and a pneumatic pump. The pump allows the tapping of two...

04 August 2024 4,662 1 View

Why is nonpoint source pollution potentially more harmful and difference between point and nonpoint sources of water pollution?

01 August 2024 1,180 2 View

How do living organisms play a role in the water cycle and why is nonpoint source pollution potentially more harmful than point source pollution?

01 August 2024 7,061 2 View