I want to do Root Organ Culture of carrot. I have tried with agrobacterium rhizogenes mtcc 532. i have tried with 8hrs, 12 hrs culture, 24 hrs, 48 hours culture.. i have collected cells by centrifugation and mixed with liquid MS mdeium i have co-cultivated it for 30 minutes. still i am not getting result? what to do. please advice

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