Hi All,
I have some compounds and I'd like to examine how they effect the expression of nitrogen transporters in maize (corn) roots. My plan is to grow corn in a defined media (i.e. Yoshida's) in varying amounts of nitrogen in the presence/absence of the compounds. The roots will be harvested, following RT-qPCR.
Can anyone direct me to information/advice (i.e. papers, reviews, assays, etc.) pertaining to nitrogen uptake via transporters in maize? It'd be even better if I could get my hands on some published qPCR primers(!). A basic literature search thus far, has suggested different types of transporters (i.e. AMTs, TIPS,) but I'd like to get more of them. I figure there's folks here who are experts in this kind of stuff.
This is a very new subject to me, so anything will be helpful!
Best,
Christian
Study finds that maize roots have evolved to be more nitrogen efficient by By Chuck Gill.Selective breeding of maize over the last century to create hybrids with desirable shoot characteristics and increased yield may have contributed indirectly to the evolution of root systems that are more efficient in acquiring nutrients, such as nitrogen, from the soil, according to researchers.About half of the yield gains in commercial corn hybrids in the last 100 years have come from improved plant genetics, explained Larry York.The other half came largely from agronomic practices, such as fertilizer use and higher planting densities.Finding new ways to improve plant nutrient uptake has global implications, according to co-author Jonathan Lynch, professor of plant nutrition, Penn State's College of Agricultural Sciences.Finding new ways to improve plant nutrient uptake has global implications, according to co-author Jonathan Lynch, professor of plant nutrition, Penn State's College of Agricultural Sciences."Creating more nitrogen- and water-efficient crops is a major goal in a world that will see population reach 10 billion people within 50 years, requiring a 50 percent increase in food production without using more land," he said. "We believe roots will be central to meeting this challenge."
Despite the dramatic yield responses of maize to N and the economic importance of this trait worldwide, relatively little is known about the genes that control nitrogen use efficiency (NUE). Efforts to understand the control of maize NUE have been limited by the complex interplay between many genes acting in both vegetative source and reproductive sink tissues. Thus, functional genomics approaches that integrate both genetic and physiological studies of whole plant N responses are both appropriate and essential to elucidating the gene networks regulating NUE.New knowledge includes estimates of breeding values for specific allelic combinations of genes that function in the uptake, assimilation, transport, and storage of nitrogen in corn tissues at developmental stages important to grain and stover biomass yield. Methods were devised for rapidly sampling tissues from large numbers of plants, and conducting parallel analyses of multiple phenotypes from the same sample.
Expression of two maize putative nitrate transporters in ...
www.academia.edu/19768506/Expression_of_two_maize...
Expression of two maize putative nitrate transporters in response to ... source of nitrogen for the vast ... maize nitrate transporters was ...
Hi Anoop,
Thank you for the response. This is very helpful.
Thanks a bunch!
Christian
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