How could i use image j to count specific cells which are labeled by two FITC ab?

how should i adjust image j to count these cells automatically not manually? i put one of my slide image in attached file.

25 December 2020 9,598 2 View

What is the basic user power allocation mechanism for achieving maximum Spectral Efficiency(SE) in co-ordinated multi-cell OFDM wireless network?

Can someone help me to realize the basic concept of user power allocation for a wireless heterogenous OFDM network where there is a macro cell and inside it some femto cells. What is the basic...

01 December 2020 4,039 1 View

Can someone help me to get nakagami fading coefficients for MIMO-OFDM channel using MATLAB?

I want to know how can we implement nakagami fading in MATLAB and get the small scale fading coefficients for this nakagami distribution in MIMO-OFDM channel. Any MATLAB code would be helpful. Are...

31 October 2020 4,375 2 View

What tests should I run for this small scale research?

25 October 2020 5,690 3 View

Why can't I see band in gel agarose while 260/280 ratio is 1.8 in Nanodrop?

Hi everyone I am trying to extract DNA from soil, 260/280 ratio is 1.8 in nanodrop, concentration is 587 ng/ul. but I can not see the band in gel agarose. Thank you in advance

18 October 2020 9,438 7 View

Does the alpha-synuclein overexpression in SH SY5Y cells have any toxic effect?

Cells are dying post-transfection. I've transfected SH SY5Y cells to overexpress alpha-synuclein, cells were overexpressed but they were dying rapidly. I've checked the overexpression, it was fine...

16 August 2020 7,518 3 View

Error while installing gromacs?

Hello all I was trying to install gromacs 2019 in an non-standard. but i countered this error: /usr/lib/gcc/x86_64-linux-gnu/5/include/mwaitxintrin.h(36): error: identifier...

16 June 2020 3,908 5 View

Number of Antennas for Massive MIMO (Commercially+ acc. to 5G Standardization)?

Currently, most of the reports show that the no. of Tx/Rx antennas for mMIMO (commercially deployed and tested) are upto128 (64T64R-TDD etc). Does anyone knows: 1. What is the number of Tx/Rx...

19 March 2020 3,987 8 View

Could you help me with simulating drag force and dielectrophoresis force in comsol by using AC/DC module?

I'm trying to apply a nonuniform electric field and create a dielectrophoretic force in a channel and put a particle in the middle of the channel. I added these 2 physics, creeping flow and...

13 March 2020 9,721 3 View

What is a good research topic for MA thesis in education or teaching English?

Im thinking to do my thesis in an international level , kind of cross cultural topic. how can I find a grant from a foreign university ?

30 January 2020 5,548 3 View

Why can't make aluminum nitride film successfully by use sputter?

We use reactive sputtering process to make aluminum nitride film, and want to make c-axis aluminum nitride film on a silicon substrate, but the crystal orientation cannot be seen with...

03 March 2021 7,975 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

How to prepare a thin polymer film by post-polymerization/crosslinking?

24 February 2021 2,362 5 View

Steps to calculate activation energy by coats & redfern method?

I'm a chemistry student and I'm researching the kinetic degradation of mixed plastic waste. I'm having trouble calculating the activation energy by the coats & Redfern method. The data that...

24 February 2021 974 1 View

How to delaminate copper in water-based anodes?

Hello everyone, I'm looking for a way to delaminate copper current collector of water-based anodes in Li-ion batteries without damaging the graphite film. I tried different materials such as HNO3...

24 February 2021 3,785 3 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

How to define the sink temperature and film coefficient?

Hi, everyone. I am considering the heat conviction during compacting simulation by Abaqus. I was wondering that how to define the sink temperature and film coefficient? Is there any references...

21 February 2021 6,197 1 View

No title

The regenerated cellulose film was prepared by zinc chloride/H2O system. The zinc chloride had been washed away in the regeneration process, and the wet film which was not dried had uniform...

18 February 2021 9,538 1 View

Droplet PCR in plastic chips always result an unexpected band, any suggestion of possible contaminations and how to improve?

I am doing droplet based digital PCR inside plastic chips. I can get the correct band, but I also consistently get an unexpected band, slightly shorter. I attached a typical gel image here....

18 February 2021 6,618 2 View