Open pulled straws which are made by manually pulling heated French mini straws usually get contaminated during processing and after that how they can be sterilized. I mean the method recommended without any deleterious effect on the quality of oocytes and embryos.
Dear Researcher
Yes while making of open pulled straws, there are chances of contamination. You can minimise contamination either by using sterile gloves when you pull the straw or after making pulled straws, wrap them in a clean cloth and Sterilize by autoclave at 5 psi for 20 minutes.
They can be sterilized by radiation or chemical or heat. Here, factors such as the structure of the material and its heat resistance are important.
It is very difficult to avoid the contamination in open pulled straws, however, can be minimised by low pressure long time autoclaving i.e. 5-7 psi for 20-25 minutes.
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