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Questions related to pH
Hi, I'm trying to start protein NMR again. When I was doing protein NMR 10 years ago, I used NMRpipe for data processing. But I think NMRpipe is not user friendly. Please teach me any other...
30 January 2022 6,576 4 View
Dear Colleagues, I am working with glioma cancer stem cells cultured in suspension as the spheroids. Generally, I used to prepare fresh, complete culture medium just before using to the cells - I...
30 January 2022 2,129 5 View
Has anybody seen the jiggling of FPLC trace like this? I have cleaned the flow cell which was hinted to be the cause of this with 0.5 M NaOH followed by 500 ml wash with MilliQ water. Still, I am...
29 January 2022 5,334 6 View
I made evaporated Cr/Au patterns through lift-off process on silica on Si. Then, I sputtered Cr on the wafer and pattered the Cr layer for SiO2 dry etch mask formation. Cr/Au was for heater on...
27 January 2022 1,030 2 View
Dear all, I am facing problem to prepare 500 ml of 0.5 M EDTA for DNA extraction. I have dissolve 93.05 g Na2EDTA.2H2O in 350 ml MQ H2O, and then adjusted pH to 8.0 with 10M NaOH. I am finding...
26 January 2022 8,081 4 View
Until now we had stored our cryopreserved tissues inside a tank with LN, but due to the high cost of this and the limiting space, we are wondering if it's possible to store those samples at -80...
26 January 2022 2,935 3 View
Hi, I got a new innoTECH pH meter, I read all manuals but they did not mention how much water I need to put in bottles. nor any mark on the bottle present, if anyone has an idea, bottles are small...
25 January 2022 7,337 4 View
I need to insert a stirrer in an anaerobic digester. biogas generating inside the digester is leaking. If I go with a long hollow pipe, in which a stirrer was inserted, then the external air gets...
25 January 2022 5,693 12 View
A pH-responsive hydrogel was synthesized and dried. It swells at higher pH and shrinks at lower pH. How can this behavour be utilized in making a biosensing device? Moreover, if superparamagnetic...
24 January 2022 5,434 1 View
I have calculated Z-average particle size from Dynamic Light Scattering (Zeta sizer). But, almost all the literature I am willing to cite for comparison has been reported diameter by volume-based...
24 January 2022 5,423 9 View
We want to perform Transient Photocurrent on the potentiostat. However, in the settings we could not find the option to perform this. Are there any supplementary softwares or hardwares needed...
22 January 2022 3,688 2 View
I need to detect the presence of Pseudomonas aeruginosa using MPN technique with asparagine broth as the presumptive test by checking for fluorescence under UV light. At what UV wavelength can I...
21 January 2022 2,013 0 View
Drones are really useful for some operations like spaying to reach places difficult by man and machine. Time was also saved. please add other applications which is cost-effective for Indian farmers.
20 January 2022 2,070 10 View
We are trying to perform IHC using fluorescence conjugated secondary antibodies for rat skin and brain tissue cryosections. What is the possibility of 4%PFA(in PBS pH=7.4) fixations to cause...
19 January 2022 8,403 3 View
Whilst many literature articles describe the use of Borate buffer (pH 8.5) for the PDL coating of cell culture surfaces the reasons why exactly seem a little harder to find. There are also plenty...
19 January 2022 6,229 3 View
Different pH of water entering in an Aeration Tank is mixed as CO2 is utilized as a catalyst. The amount of CO2 as catalyst is unknown.
19 January 2022 6,908 7 View
My experiment needs anhydrous orthophosphoric acid and we only have a 85% aqueous solution. Any method to dry it? Most of the literature I found deals with purification from metal ions present in...
19 January 2022 5,632 1 View
A pH sensitive hydrogel is embedded with superparamagnetic nanoparticles. This, in response to pH change, swells or shrinks. Also, the hydrogel has now magnetic sensitivity as well as for pH. How...
18 January 2022 1,486 2 View
I dissolved a poorly soluble drug molecule into a solution of 0.1N HCl and β-cyclodextrin, but when I increased the pH by adding NaOH, the solute returned from the solution and turned the solution...
18 January 2022 438 3 View
how chlorides and sulphates interfere the removal of ammoniacal nitrogen from industrial waste water
18 January 2022 8,719 7 View
Hello. I am considering how to statistically compare 3 datasets (plants from different proveniences) consisted of measurements of leaf damage 30 leaves each provenience. I measured the damaged...
18 January 2022 9,758 4 View
Hello. I am currently trying to make a buffer for protein expression. According to the article, I am to use a buffer with 50 mM NaxPO4, pH 8 (and 0.5 M NaCl). How do I make the NaxPO4 buffer?...
17 January 2022 4,919 1 View
Whether the pH of lysis solutions (while RNA extraction) need to be modified as per the cholesterol content in the cell's plasma membrane?
17 January 2022 4,320 3 View
I would like to know your experience on the potential impact of the extent of dilution of a micelle formulation on the variability of Zetasizer parameters (surface charge, PDI and size). Thanks
17 January 2022 4,482 5 View