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Questions related to pH
As stated in the product information file, I dissolved the GSK2606414 molecule in DMSO at 10mg/mL and stored it (30 uL aliquoted) at -20 degrees C. However, when I wanted to store the leftover...
09 April 2024 758 0 View
I have recently published a paper in a Scopus-indexed journal. Is DOI essential? How long will it take to get DOI ? Thanks in advance
09 April 2024 4,086 3 View
spectrophotometric method Si
08 April 2024 4,605 1 View
Hello！ When constructing the plasmid, I used the same promoter. However, the direction of each expression cassette promoter in the obtained plasmid is the same, and the plasmid with the opposite...
08 April 2024 9,016 1 View
The ability of microorganisms to degrade complex organic compounds in environmental bioremediation processes is influenced.
06 April 2024 1,714 1 View
Dear Research Community, I am encountering a significant hurdle in my research involving enzyme inhibition testing. The inhibitor I am investigating exhibits solubility exclusively in DMSO,...
06 April 2024 1,176 4 View
Initially, I conducted maceration with a 70% ethanol solvent for 3x24 hours to obtain the thick extract. For the AgNP synthesis, the thick plant extract needs to be dissolved using deionized...
05 April 2024 6,884 4 View
After electrospinning 17 wt% cellulose acetate, diacetylation was performed in a 0.05M aqueous NaOH solution. After that, it was washed several times with distilled water and dried naturally in a...
05 April 2024 6,934 0 View
Hi All, I am trying to amplify mitochondrial 16S gene for marine snails (Calliostomatidae) and other vetigastropods, but I only get primer dimers or nothing on the gel. These primers have worked...
04 April 2024 8,729 4 View
Hello everyone, I am trying to use the new type of reaction present in Aspen plus V14, known as "pyrolysis." I have tried to simulate pyrolysis of HDPE, by entering the kinetics suggested by...
04 April 2024 2,081 0 View
I am looking to do Immunofluorescence experiments with a myoblast cell line and was wondering if anybody has had success coating glass coverslips in Matrigel?
03 April 2024 6,165 3 View
Why does the AgNP size measurement appear smaller in DLS (13nm) compared to TEM (43nm)? Attached is a TEM image displaying the nanoparticles with an approximate diameter of 43nm, while DLS...
02 April 2024 2,762 5 View
How climate change affects hydrology and water resources of Indian River basins and climate change affect thermohaline circulation?
01 April 2024 8,633 4 View
I use 0.25% trypsin (2.5g/L) as a positive control in my protease assays. I'm trying to measure the stability of different proteases overtime and at different temps. When I measure the...
31 March 2024 8,481 0 View
I deposited a polymer dye on cotton fabric. It seems it is covalently attached. But i am confused what can be the possible mechanism for covalent interaction between the polymer and fabric?
30 March 2024 2,072 1 View
AI is coming fast, and we have to adapt.
30 March 2024 2,305 1 View
SFC refers to the Solid Fat Content which is generally estimated using NMR. I would like to ask about the possible ways to estimate this value using the DSC program? Please suggest.
30 March 2024 7,611 0 View
Hello Honorable community! I am looking for a free of charges and an indexed journal in the field of microbiology with an impact factor between 1 and 2. Do you have any experience?
28 March 2024 4,905 3 View
Hello all, I'm managing a very large scale project, which benefits from many automated processes to produce and image slides stained with a fluorescent conjugate. Surprisingly, the biggest...
28 March 2024 7,909 2 View
I’m currently using a protein (36 kDa) which needs to be unfolded during a labelling reaction. unfortunately the protein precipitates completely upon denaturation with EDTA which chelates the zinc...
28 March 2024 8,852 2 View
I will plan to measure Magnesium isotope in water and soil. Can anyone help me to find a relevant lab to measure Mg isotope and their sample requirement (sample quantity, preservative techniques,...
26 March 2024 1,209 1 View
What is the role of algae as a biofuel and potential of microalgae for sustainable biofuel production?
25 March 2024 8,744 2 View
I am conducting a cell viability assay on a large sample of adherent cells unable to be distributed into a traditional sized 96 well plate. How would I analyze this larger sample? Would an ATP...
24 March 2024 8,238 1 View
I m currently doing a research on Data mining in Digital marketing and will like to get your opinion 1. The effects of mining and its impact in digital marketing 2. Does mining artificially alter...
24 March 2024 4,176 1 View