pH

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I am having some problem regarding crispr protein?

I have inserted crispr vector in BL21 competent cell with ampicillin resistance and after induction with IPTG for protein ...no cas protein band was obtained using SDS page.. not even near to its...

28 February 2024 8,229 4 View

Is my recipe of loading buffer for SDS-PAGE correct?

Hi Everybody. I'm senior in a University in Vietnam. This is fisrt time I do SDS-PAGE, so I want to ask about the correction of my loading buffer recipe for SDS-PAGE as a following file. In...

27 February 2024 7,544 3 View

What will be the best methods to remove enzymes from laboratory waste water?

We are using some enzymes to test in the laboratory. Trying to figure out the best method treat the waste water. The actual chemical we using are testing with enzyme based drain cleaners. Need...

27 February 2024 871 6 View

This is my Western blot data.The last band that has pure ADH on it is supposed to show a single band like other lanes .WHy does it look so?

The detailed Western Blot procedure The membrane is first rinsed briefly with 20ml of TBS, followed by a 30-minute incubation, shaking with 10ml of Blocking Buffer. During this incubation, the...

27 February 2024 1,406 2 View

What should we do to grow hairy roots that have appeared?

Hairy roots can be seen well on leaf and stem explants, but these roots do not grow.

27 February 2024 6,316 1 View

How do you confirm the antibacterial activity is due to phytoconstituents and not due to solvent residues?

26 February 2024 9,614 1 View

How to remove DCU and its remaining traces from Steglich esterification?

Firstly, the compounds that I want to synthesize are alkyl gallates (ethyl gallate, butyl gallate, and hexyl gallate). The overall reaction: Gallic Acid + Alcohols + (THF, DCC, DMAP, 0 Celsius)...

26 February 2024 2,146 3 View

How would go about aswering this question?

You’ve been appointed as a marine scientist to examine whether farmed fish fed with one type of food (type A) will produce more offspring than farmed fish fed with a different type of food (type...

24 February 2024 688 0 View

How Bradford method protein result can be converted into percentage protein? I am using 5 ml extraction buffer to extract the protein from leaves?

Regarding protein estimation

23 February 2024 4,679 1 View

Experience in vacuum removal of water and ethanol?

I am trying to dry my extract using a make-shift vacuum setup with a flask (containing the sample), a solvent trap and a vacuum pump. My heat source would either be a water bath. Can anyone share...

23 February 2024 2,401 1 View

Is there any other way of imposing 'no reflection' boundary condition than the popular PML (Perfectly Matching Layer) method in FE setting?

In the context of acoustic wave propagation through heterogeneous media, PML is commonly employed technique to impose 'no reflection' boundary condition. I'm interested in knowing whether there...

20 February 2024 4,552 1 View

I am doing a froth fotation to raw graphite. after the flotation how can i remove the flotation reagent ?

(i am using kerosene as collector and butanol as a frother).

19 February 2024 7,787 2 View

Why does my clay contract after glycol treatment for XRD analysis?

Recently in my lab I’ve been trying to expand some Muscovite following two different protocols. I use XRD to verify the d-spacing after the treatment without ethylene glycol and with ethylene...

19 February 2024 3,579 0 View

In serum proteomics, we add 15% TFA to stop trypsin activity. How does TFA stop trypsin activity?

serum proteomics

18 February 2024 1,047 3 View

How does generative AI impact creativity?

17 February 2024 5,641 4 View

Which SDS for electroplating?

I have three SDS classifications which are Sodium dodecyl sulphate, ACS reagent, ≥99.0%, Sodium Dodecyl Sulphate, >85.0% (T), Sodium dodecyl sulphate, >97.0% (GC)(T). Could somebody tell me...

15 February 2024 7,280 1 View

Multimaterial laser cladding - suggestions?

I'm currently working with Michael Ukaenwe on ABA cladding, a new variant of laser cladding recently developed at The University of Nottingham with John Powell and Kóti Dániel. We're now...

14 February 2024 6,556 0 View

What are the steps used for the separation of dyes from textile effluents ?

How to remove dyes like rhodamine and methyl violet from the textile effluents by electro coagulation and electro oxidation?

13 February 2024 6,636 1 View

How do I upload my publications directly from database?

how do I upload my publications directly from database?

13 February 2024 2,581 1 View

Microfluidic chip- how to prevent hydrogel peeling and retraction?

Hello! I inject 1 mg/ml Collagen + matrigel mixture in one channel of my device and 2mg/ml collagen in the adjacent channel. Two channels are separated by microposts. I somehow see hydrogel...

12 February 2024 8,147 1 View

What are the key factors influencing the pricing dynamics of agricultural commodities in global markets?

I need ask - What are the key factors influencing the pricing dynamics of agricultural commodities in global markets?

12 February 2024 7,157 2 View

How Do I Calculate Gravimetric Capacity?

Dear All, I have been trying to use BioLogic Electrochemical Equipment to investigate my gravimetric capacity. I am aware that the capacity would be divided by the mass of the active working...

12 February 2024 4,773 1 View

Mineral Oil Electrical Conductivity ?

What kind of instrument do I need to measure the electrical conductivity of a very low conductive fluid such as mineral oil?

11 February 2024 4,485 1 View

What do I do when I don't see density in my gromacs energy selection?

I tried to proceed with NPT as usual in protein MD simulation in water and get the density for the whole system. However, I don't see any "density" items in my "gmx energy" selection. The same...

08 February 2024 6,825 0 View

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