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RAW264.7 cells turn yellow within 2 days, is it solely because of mycoplasma, or could it be linked to other factors?

I seeded 2x10^6 RAW264.7 cells in T-75 flask. Normally, they will have a confluency of 90-100% within 2 days (no change in cell culture media color). However, recently, the cell culture media in...

27 August 2024 6,031 0 View

How to derive hydrodynamic equations from Heisenberg's equation of motion?

People usually derive hydrodynamic equations from the Boltzmann equation. How to derive fluid mechanics equations from Heisenberg equation?

27 August 2024 8,110 1 View

Does anyone know where I can obtain Pseudomonas syringae hrcC- mutant strain that is deficient in Type III secretion system?

If you know of any researcher (ideally within the US) who has the Pseudomonas syringae hrcC- mutant strain in his/her/their collection, please let me know. I would like to request for it as soon...

26 August 2024 9,000 1 View

How to Calculate wt% of Fe, P, S, and Li from ICP-OES Data?

Hello everyone, I’m currently working on analyzing ICP-OES data and I need to calculate the weight percent (wt.%) of Fe, P, S, and Li in two LiFePO4 samples from the results. However, I’m...

25 August 2024 7,623 6 View

How can biobanks be integrated into pharmaceutical research and development to enhance personalized medicine and drug discovery?

"The integration of biobanks in pharmaceutical research has opened new avenues for personalized medicine and drug development. By storing and managing biological samples linked to extensive...

24 August 2024 1,390 2 View

What are some best known methods to modify HDI (Hexamethylene diisocyanate) to make it more reactive for uv curing of ink?

Hi, I have made a binder polymer resin for uv curing of ink. The viscosity of binder polymer is between 700 to 1300 cps. Basically I disperse the inorganic pigments in the binder polymer along...

23 August 2024 9,792 6 View

Why thermal conductivity of nematic liquid crystals doped with MWCNTs decreases at higher room temperature?

why thermal conductivity of nematic liquid crystals doped with MWCNTs decreases at higher room temperature?

23 August 2024 1,690 1 View

What are the differences between interlaminar and translaminar fracture toughness?

Translaminar implies the breaking of fibres, whereas inter- and intralaminar refers to whether the cracks grow in between the plies or through the thickness, respectively. Need clear explanation !!

23 August 2024 3,728 1 View

Do you know best Ag deposits in which conditions can be formed?

I want to know more about Ag deposits.

22 August 2024 7,995 1 View

What do you do if you read a published paper, then you find out a piece of your work is there but you were never included on publication ?

what do you do if you read a published paper, then you find out a piece of your work is there but you were never included on publication. while you were told that the information was irrelevant,...

21 August 2024 9,604 3 View

Hello Colleagues in my area!! I am in need of doing PhD in Epidemiological Modelling of infectious disease. Would you please help me?

Hello Comrades, I am indeed a scientific researcher in a domain of disease modelling in Epidemiology using Mathematical Models. Anyone in this area, and possibly having funds in similar projects,...

21 August 2024 7,979 0 View

What are the ethical considerations in applying gene editing technologies to agricultural crops?

the ethical considerations used in applying gene editing technologies to agricultural crops?

19 August 2024 6,984 1 View

Critically analyse the factors that contribute to food insecurity and how these can be ameliorated?

using the following articles. Jacobsen, S et al (2013); Qaim, M. & Kouser, S. (2013) ; Zewdie, A. (2014) ; Candel, J., (2017).

16 August 2024 7,734 0 View

What are the long-term impacts of incarceration on youths' developing brain?

I want to explore the long-term effects of incarceration on a youth's developing brain. I also want to explore research that looks critically at incarceration and punitive measures as the primary...

13 August 2024 1,452 0 View

Nano Orange protein quant on the Quantus fluorometer?

Is anyone here using the Thermo Fisher Nano Orange kit for protein quantification, and detecting the fluorescent signal on the Promega Quantus? We are struggling to make sense of the standard...

06 August 2024 9,164 3 View

How can i study the carboplatin using spectroscopic technique, viz Raman and FTIR spectroscopy?

I'm working on carboplatin which i bought from local pharmacy. It is a Carboplatin injection IP 150mg/15ml which is sterile aqueous solution for intravenous use. Unfortunately, I cannot able to...

05 August 2024 595 3 View

How do I convert cellulase enzyme 10,000U/g to mg/g fresh forage or mg/kg fresh forage?

I would like to know the minimum amount of Cellulase enzyme that could be used for silage making.

04 August 2024 3,290 1 View

What is the cycling of matter through ecosystems and how energy drives the cycling of matter in photosynthesis and cellular respiration?

03 August 2024 10,161 2 View

How can AI-driven personalized learning balance individual instruction with fostering social and collaborative skills?

This question tackles the complex issue of ensuring that while AI can tailor educational experiences to individual needs, it doesn't neglect the equally important aspect of fostering social...

03 August 2024 5,075 3 View

How about Subsequent citations?

Today I received notification of 3 more subsequent citations but no information about the citing papers Hmmm

03 August 2024 2,729 3 View

How to correctly use the UTE and ZTE pulse sequences in Bruker's ParaVision software?

I am using a Bruker 600M solid-state NMR spectrometer with a Micro 2.5 microimaging system. The test sample is a tube of 1M LiCl aqueous solution, and the nucleus detected is 1H. I am trying to...

02 August 2024 10,236 1 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

01 August 2024 908 2 View

What are the factors that causes the sample to have higher efficacy at lower concentration?

We conducted an antibacterial study of a plant extract. Varying concentrations of crude extract were subjected to microbroth dilution assay. The result showed that only the lowest concentration of...

01 August 2024 9,226 3 View

Where to find a gene list for CRISPRa/i library screening of regulatory factors that affect pathogenic Th17 differentiation in PBMC?

I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...

01 August 2024 2,893 0 View

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