Contact experts in pH to get answers
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Questions related to pH
I want to build some standard curves for a large number of volatile compounds. I am hoping to combine different classes of compounds into one mix in order to reduce the number of instrument runs....
18 April 2022 4,780 1 View
Hello, I have performed NaCl extraction of a nuclear protein and run it on RP-HPLC with a mobile phase of acetonitrile in 0.1% TFA in water. I had repeatedly observed two tall peaks around minute...
14 April 2022 1,761 0 View
I am working on polymeric beads with different surface treatements to improve their hydrohilicity and thus biocompatibility. What paramters are important for comparative zeta potential...
13 April 2022 3,495 2 View
Recently I noticed that air bubbles constantly appeared from my pump and keep clogging my columns. 【Solvents】 Solvent A =sodium acetate buffer (0.05 M, pH 6.0) in water containing 5 mM β-CD...
12 April 2022 5,008 5 View
Preparation 1-aroyl benzofuran from salisaldehyde
12 April 2022 4,848 5 View
Dear Research Community greetings, I'm working on milk quality for my Ph.D thesis and I need to know what is the protocol which shall I follow to detect the antibiotic residues in raw cow milk...
09 April 2022 6,726 9 View
I am trying to prepare the encapsulated PCMs in which the core is paraffin wax and the shell is Silica using the sol-gel method. I am following this paper; Synthesis and Characterization of...
05 April 2022 551 4 View
I am planning to do a lot of ChIPs following 'Chromatin techniques for plant cells Bowler 2004' and thus I am planning on stockpiling a lot of stock solutions and premade ChIP buffers. I'm having...
02 April 2022 1,020 1 View
Greatings, I am looking for a manual or book that includes the metodology to determine microbial biomas and fungal to bacterial ratio using a microscope. Thank you in advance.
02 April 2022 4,345 0 View
Please give your advise on soil chemical mineral requirement like NPK, Ca, Mg,pH,EC, Micronutrient etc
31 March 2022 553 2 View
Recently, we’ve started testing the ability of previously isolated bacteria to fix nitrogen. For that reason, we have prepared a solid N-free medium (namely NFb)...
29 March 2022 8,777 3 View
Dear all, I'd like to know your opinion about an issue I'm facing during the isolation of adult rat cardiomyocytes (CMs). The protocol I've been following is as follows: - Rats are anhestetized...
28 March 2022 3,927 0 View
How this tailing can be decrease for my 5 HMF detection?
28 March 2022 4,881 4 View
Structural equation modelling is key to understanding the internal workings of an ecosystem. I wanted to know if it's possible to build a SEM utilising data such as 1. Soil physical and chemical...
27 March 2022 5,884 2 View
I would like to make a complex of manganese with edta in water. Until now i just tried 0.5 mM of the acid EDTA and 0.5mM of MnCl2.4H2O in 100 mL solvent water, put the reaction 37°C for 3 hours...
26 March 2022 4,458 3 View
I was trying to measure aspartyl protease activity under acidic pH using 1% azocasein dissolved in Tris-HCl (pH 8.0). However, azocasein precipitates out in acidic pH when I add enzyme solution in...
24 March 2022 3,712 2 View
We are trying to determin the pH of acetic acid solutions in the range of 95 to 75 vol%. The pH meter shows strongly different values for 75 (pH = 0.5) and 95 (pH = -0.5) vol% from the calculated...
24 March 2022 1,994 3 View
We want Potassium doping along with MnO2 doping on our support. I would like to ask whether KMnO4 be used for simultaneous doping of Potassium and MnO2.
24 March 2022 4,623 3 View
A mixture of 0.4M copper sulfate and 4M lactic acid has been prepared, and then 4M sodium hydroxide solution is added to adjust the pH, but the more sodium hydroxide is added, the more the...
23 March 2022 1,432 3 View
I need to make a 5mM solution of kynurenic acid to use in organotypic cell culture. I tried to first make 5ml of a 1M solution in 1N NaOH and then make the volume to 50ml by adding 45ml of H20....
21 March 2022 5,184 2 View
Last year we started to use DreamTaq polymerase by Thermo Fisher in our lab. Comparing with the usual recombinant Taq polymerases by Thermo and NEB I have an impression of its poorer performance...
21 March 2022 5,038 3 View
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18 March 2022 7,602 1 View
When using buffers to maintain pH for sorption isotherms using natural waters, we are seeing a pH shift of 0.25 over a 24 h reaction. Are there ways to keep the pH more uniform than this?
17 March 2022 9,336 2 View
Hello everyone, I'm currently trying to expand human cells in a 2L cell culture bag (plastic), but I have problems with the medium getting to acidic - even when no cells are involved - and the CO2...
17 March 2022 9,254 3 View