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Questions related to pH
when pH,amount of catalyst and concentration of the dye are considered
16 January 2022 9,376 4 View
E.g. is there a particular soil trait (pH, chemical content, concentrations of carbon or nitrogen, loamy vs. sandy, etc.) that is associated with higher populations of diazotrophs? I'm more...
14 January 2022 1,395 4 View
I would want to apply a Genome Shuffling (GS) protocol to my strain of Cupriavidus necator, but I am reading that GS is mainly based on protoplast fusion. Is it possible to perform it on...
14 January 2022 989 1 View
How can I analyze EPR (electron paramagnetic resonance) data for nanocomposite material?
14 January 2022 9,037 2 View
I wanted to see the interaction between collagen and a therapeutic agent at the physiological condition in vitro. Is there any way to dissolve (lyophilized) collagen type I in interstitial fluid...
13 January 2022 4,053 3 View
Hi, I have made a purification under denaturating condition using urea. Any suggestions for protein refolding following elution? Thank you in advance.
12 January 2022 9,901 5 View
Kindly give the names of crude oils that can be used for waste water treatments.
11 January 2022 8,401 21 View
Can Var% explained in the random forest replace R Square? And is Var% explained similar to the result of cross-validation?
11 January 2022 5,775 3 View
I was going through a study where the author mentioned that, they chose the test concentration of 0.5mg/l where the solubility of the substance was 0.01mg/l. There they mentioned that the test...
10 January 2022 5,554 2 View
Hello everybody I had been doing semi-dry transfer in past few days and have experienced a persistent problem. I set-up my semi-dry transfer as follows: 1. Run a 12% SDS-PAGE gel under normal...
09 January 2022 4,068 2 View
To Know the Trace elements concentration in carbonate and phosphorite minerals separately.
09 January 2022 9,929 1 View
Soil is one of the most important life sustaining materials on the earth. It is an essential medium for plants growth that initiate the ecosystem and provide food, either directly or indirectly,...
07 January 2022 4,818 33 View
How to Construct AFM and FM magnetic ordering in CASTEP code implemented in materials studio software? In rutile TiO2, I want to calculate the ferromagnetic coupling and antiferromagnetic coupling...
05 January 2022 3,890 0 View
Hello, I'm doing the lift-off process for the deposition of Cr3nm /Pt 200nm on a Pyrex substrate. I'm using a LOR3B photoresist and S1818 resin and the developer is the MF319. For the lift-off i...
05 January 2022 7,754 4 View
How good is citric acid as the nitrogen source as compared to ammonium sulfate in the growth of bacteria?
04 January 2022 4,073 4 View
Hi friends I have one protein that have difficult process(use 2me, change pH up to 11.6 and some changes in induction step) to solublizing it in supernatant. after change pH to 11.6 my protein...
02 January 2022 5,923 5 View
Neonatal cardiomyocyte is the sensitive primary cell line. Can a researcher cryopreserve the cell line for future experiment?
31 December 2021 4,158 3 View
I have noticed that copper oxidation on copper nanoparticles often coincides with aggregation of nanoparticles into ,much larger clump in my experiments. I was hoping for some insight of...
29 December 2021 2,320 3 View
Whether lPS-induced inflammatory can induce mature il-1β secrete?
24 December 2021 2,059 3 View
Hi everyone, I have been trying to calculate the Km value of a recombinant HCV NS3/4A protease using a FRET substrate, the cleavage of which can be detected at 500 nm using excitation wavelength...
23 December 2021 4,703 14 View
Phosphoric acid is in the form of H3PO4 remained free while manufacturing of SSP fertilisers. So far Quality of fertiliser is concerned the free phosphoric acid should be less than >4% as it...
20 December 2021 9,893 3 View
I have been making labeling medium for NMR work where I add the following: K2HPO4 12 g/L KH2PO4 9 g/L 15NH4Cl 3 g/L NaCl 2.5 g/L ISOGRO®-13C,15N, Powder 1 g/L 13C-Glucose 4 g/L MgSO4...
17 December 2021 3,062 1 View
For a biosorbent, effect of pH variation from (3 ~ 10) on % removal efficiency of Nitrates from water is recorded. Max. removal is at pH 7. Does this wavy graph make sense?
15 December 2021 2,654 3 View
Hello, I am trying to purify a recombinant his-tagged protein (MW: 29.5 kd, pI:5.7, mostly composed of beta strands). It has been expressed from BL21(DE3) competent cell. I am using 50 mM Tris,...
15 December 2021 1,053 5 View