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Questions related to pH
I was checking an old protocol I used to do for genomic DNA extraction, and it says "Tris-acetate 40 mM pH 7.8", but I can't remember how it is made, does it mean that I have to make a 40 mM Tris...
17 March 2022 5,804 3 View
Hello everyone, I am doing some antioxidant assays and for that I need to prepare acetic acid buffer with pH 4.5, I searched a lot about this but can’t found an answer which I can understand...
17 March 2022 7,194 3 View
I prepared LB media with different heavy metals in them separately and they solidified naturally but when I added arsenic to one of the media, it was not solidified. What can be the reason behind...
17 March 2022 9,291 2 View
I need help for this identification. I think it's a cyanobacteria (maybe an Oscillatorial?) but maybe it's a bacterium. This taxon is filamentous and the dimensions of the cells are: * Diameter =...
16 March 2022 1,992 2 View
I am trying examine the small components in faecal virome by ultrafiltrating a fecal suspension to remove the bacteria and afterwards staining with fluorophores (eg. SYBR Gold). My primary...
16 March 2022 4,813 0 View
Please guide me for calculation of carbon sequestration per year?
16 March 2022 572 8 View
What are the main criteria that must be taken into account in order to choose the appropriate place for the completion of the technical landfill center for urban waste in the Hodna region, taking...
16 March 2022 8,571 4 View
why does the carbon to nitrogen ratio be important in preparing compost for plant growth? if it is an important parameter to be analysed, what is the standard ratio that should be maintained?
16 March 2022 9,682 11 View
Dear all, I encounter problems of precipitation when I am trying to reproduce a known culture media with buffers like HEPES ; PIPES or MOPS I am preparing a minimal synthetic medium for the...
15 March 2022 6,799 3 View
I have designed 15-Runs with different substrate concentration, pH, and temperature parameters in order to produce hydrogen from cow dung + algae through dark fermentation technique. As per the...
12 March 2022 9,206 3 View
Has anyone ever seen autofluorescence in the nucleus of tomato plant leaves? Particularly in the GFP channel? This signal seems to get brighter with age and not entirely consistent. Wondering if...
09 March 2022 5,214 2 View
Dear Sir/Mam, Should I check model fit for secondary data. Through unit root test the series is stationary. So is that enough and can i proceed with the analysis. Kindly help.
08 March 2022 8,500 2 View
Hello, i want to isolate Lactobacillus from different food samples and measure their antimicrobial effects on pathogens .. the problem is that i can't find any MRS agar that is not expired ... can...
06 March 2022 7,572 4 View
I have sodium phosphate dibasic heptahydrate and sodium phosphate monobasic monohydrate and also the anhydrous forms. I want to know the preferred solutions to use and method of preparation
04 March 2022 8,177 2 View
I am performing immunoprecipitation (pulldown) of proteins using Ni-NTA agarose resins. My test control showed that the target/prey protein binds to the Ni-NTA agarose resin in the absence of the...
03 March 2022 7,840 2 View
Hello, I am performing a batch experiment of producing biogas from cow dung and chlorella. After performing CHNS, I found the C:N ratio of cow dung to be 12.34 and chlorella would be around 5. I...
03 March 2022 2,478 3 View
I'm doing microbiological monitoring of highly purified water.
02 March 2022 1,551 3 View
Hello! To measure protein concentrations in my sample, I use the Nanodrop2000 and the protein A280nm method. However, I am not sure how to interprete my results. For example in the picture shown...
01 March 2022 3,388 3 View
Hello, maybe anyone has an idea why this bands looks like below? This is native page for basic proteins (acidic gel) with reversed electrodes 8 x separating gel buffer: 2,2 M CH3COOH pH...
28 February 2022 4,954 3 View
I went through few papers, in so,me they are saying that acidic pH help in wound healing and in some they are saying that neutral to alkaline is better for wound healing. So I am confused. It...
27 February 2022 276 2 View
Can anyone list out the advantage and disadvantages of water pollution remediation technique/ method in a broad and detailed approach?? For example UV treatment / Electrochemical/ biological...
27 February 2022 7,881 11 View
My sample is montmorillonite and Fe3O4-montmorillonite.I want to know the zeta potential of both. How do i prepare the sample before introducing to zetasizer. My work is adsorption of pesticide at...
26 February 2022 9,911 1 View
Is there any reason of pH increasing in an anaerobic treatment system?
25 February 2022 7,451 3 View
Which are the best methods for the measurement of soil pH?
24 February 2022 5,560 4 View