Hey everyone, so I am trying to perfect my procedure for the clot retraction analysis and I am struggling to keep the clot on the meniscus line. I am treating my cuvettes with pluronic to prevent the clot from sticking on the sides and my pipetting is precise enough so I am making sure that I don't pipette way into the solution. The concentration of CaCl2 and thrombin is 2mM and 0.2 U respectively, this has been changed a lot with time. I worked with 20mM and 1 U, 10 mM and 0.5 U and now I am doing 2 and 0.2. The total volume is 500 ul (which was once decreased to 300 with 10 mM and 0.5 U Cacl2 and Thrombin respectively). Many different papers are using Triton X as a lubricating agent but I used Pluronic in my case.
It would be great if anyone could help me solve this.
Dear Prarthna Kashyap
It sounds like you've already made several adjustments to your procedure to address the issue of clot retraction, but you're still experiencing difficulties. Here are a few additional suggestions you might consider:
By systematically evaluating these factors and making adjustments as needed, you can optimize your clot retraction assay to achieve reliable and reproducible results.
Sincerely
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