I have used the DLS machine (Zetasizer Software) to measure the size of micelle prepared. For the first 2 runs the quality report shows "Good", while the 3rd measurement shows as "Too Polydisperse" for thee same sample; the Z-avg value(26.48,26.64,27.93) and PDI value(0.558,0.563,0.502) are as stated. (The 3 runs are automatically done by the machine.)
In my experience, encountering discrepancies like this in dynamic light scattering (DLS) measurements often indicates potential issues with sample integrity or experimental conditions.
It's not uncommon for variations in particle size distribution to arise from factors such as aggregation, degradation, or contamination. By identifying these potential sources of error through careful investigation,
researchers can gain valuable insights into the reliability of their data and take necessary steps to address any underlying issues.
- Badges
- Science topic
- Similar topics
- Biological Science
- Biochemistry
- Biochemical Techniques
- Analytical Chemistry
- pH