Dear Team Fungi,

We would like to identify root fungi from Vitis vinefera via metabarcoding. The methodology is established, we have had good results with other root samples using the isolation kit 'innuPREP DNA/RNA MiniKit' and the standard primers gITS7ngs/ITS4ngs with 49 °C annealing temperature. Unfortunately, we do not get any bands from Vitis roots, no matter what we try (e.g, adding BSA or a temperature gradient). Does anyone have any ideas on how to modify the DNA isolation or PCR to eliminate the potential interfering substance in Vitis roots? There must be something in there that interferes with the PCR...

With desperate regards

Kai