Methanol and ethanol plant extracts of lemongrass, centella asiatica and moringa oleifera not showing activity in the varied concentration of lower to higher concentration 300mg/ml, 500mg/ml and 400mg/ml from the stock taing 50 100 150 and 200 microlitre) using disc and agar well diffusion method. tested organism is E.coli, Bacillus and staphylococcus aureus. Even after the review of literature of the related paper and following same methodology we not getting results please let me know where we lagging?
Thanks.
Hey Ashwini Murthy !
Problem might be somewhere with the preparation of extracts and the protocol.
the stocks of 300, 400 and 500mg/ml are very much higher concentrations and it is very surprising that you are not getting any activity. I think you should revise your protocol and then from any of your stock conc. (e.g. 300mg/ml) prepare the sub concentrations and test.
Shashank Kashyap thank you for the answer will try that once again and before also we tried from stock concentration doing sub concentration there also no proper results. yeah will try again
Try using lower concentrations like 10mg or 20mg initially. Sometimes, higher concentrations yield no results, whereas lower concentrations can provide better outcomes. Ensure you properly filter your extract before conducting any biological assay.
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