I have 30 days old spheroids (organoids) which originate from stromal vascular fraction.
They are usually big in size, growing in 96-wells.
I can use TrypLE, Accutase, Coagenase, Trypsin, or Hyaluronidase.
Do you have any experience or recommendations?
Thanks
Hossein Abolhassani
It depends on how compact they are. I would add Trypsin to the wells and put them in the incubator for 10 min. Then, pipet them gently to obtain single cells. If The spheroids are still dense after pipetting, I would repeat the steps (incubating and pipetting) 2 or 3 more times until I get single cells.
Bets,
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