Are the binding constant and binding affinity is the same thing in both fluorescence quenching or fluorescence increment? What is the meaning of getting high or low binding constant (kB), derived from the modified stern Volmer equation: log [F0-F/F] = log [Kb] + n*log [Q] in ligand-DNA interaction study by fluorescence spectrometry?
Does high binding constant means strong interaction between ligand and DNA?
I believe that Kb in this equation is the inverse of the equilibrium dissociation constant, with units of inverse concentration (e.g. L/mol). Therefore, the higher the number, the greater the affinity of binding.
I saw a reference for the equation, but the paper is not open access: Article Thermodynamics of Protein Association Reactions - Forces Con...
- Badges
- Science method