I am in the process of identifying specific small RNAs from raw data obtained through total small RNA sequencing. Could you guide me on aligning these particular small RNAs with the gene sequence of interest within the bulk dataset?
How and where can I publish my science pamphlet so that everyone can cite it? I dont want to pulplish it in journal due to reviewing process, I just want to record it and allow others to cite...
20 July 2023 6,404 0 View
Evaluate the use of repository services by researchers.
08 June 2023 1,946 4 View
select a map in sumo and after run insert it in omnet++ for simulate a routing protocol?
05 January 2023 8,746 0 View
hi There are not many publications I could find about wind-geothermal hybrid systems. I want to know what the problems are related to this issue. I need an authentic resource that discusses the...
15 July 2022 7,447 3 View
Hi every body, I want to fabricated a supercapacitor electrode with Ti3C2Tx (MXene) on carbon fiber. my question is : How can I encapsulated carbon fibers to avoid capillary effect of the...
12 July 2022 3,843 0 View
Dear colleagues, we are working on an extensive meta-analysis and are trying to do subgroup analysis via Stata 16. When we do subgroup in stata16 by this command: . meta summarize, random eform...
07 September 2021 1,472 2 View
I have another three questions about theoretical computations in photovoltaics systems. I was wondering if you could help me with. 1-If the module efficiency written in a catalog is 18% and the...
16 April 2021 756 3 View
hello I have three questions about theoretical computations in photovoltaics systems. I wonder if anyone skilled in this area could help me. 1-If the module efficiency written in a catalog is 18%...
14 April 2021 1,233 6 View
The purpose of these calculations is to evaluate the performance of a commercial photovoltaic panel with the specifications of Table 1 under environmental conditions in Table 2, which was obtained...
09 April 2021 9,869 9 View
I want to know in the cold weather for improving performance and immunity which nutrient is added to the diet.
22 November 2020 2,454 4 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?
11 August 2024 5,138 1 View
I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...
10 August 2024 8,539 5 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...
08 August 2024 9,882 2 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...
06 August 2024 807 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
Hi I am working on data driven model of the microgrid, for that, i need the reliable datasets for the identification of MG data driven Model. Thanks
02 August 2024 5,748 4 View