Our bacterial protein is located in cytoplasmic membran not in cytoplasm?

08 May 2025 0 10K Report

The bioinformatics and computational analysis predicated epitope now should i confirm the predicated epitope in wet lab or need to check the whole protein?

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Science method

More Khyber Shinwari's questions See All

How we will calculate sugar-acid ratio?

if we have TSS value in degree brix (i.e. 12) and total sugar value (Reducing+non Reducing) i.e. 8, and percent acidity value i.e. 0.7%..... what will be the value of sugar acid ratio?

21 November 2023 8,560 0 View

How to run VECM Granger causaliyt test?

I want to apply long-run and short-run Granger causality test for time series data. The one I find in Eviews is very simple and did not show the direction of causality as well as F-statistics and...

04 June 2019 2,063 10 View

How do i do statistical tests?

I have been looking at long term exposure of cyclotrisiloxane on MCF7 cells. I have data on 4 weeks and now I have to do some statistical analysis. I used ethanol as control for this experiment....

20 January 2016 4,772 10 View

Published ethnobotanical uses of Bergenia ciliata

Berginia has some valuable compounds. I am looking for validation from published literature regarding its usage.

15 July 2013 1,561 3 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Seeking Software Recommendations for SELEX NGS Data Analysis?

I am looking for software to help analyze SELEX NGS data, including alignment, sequence enrichment, and other related tasks. Can anyone recommend suitable tools or software? Best wishes, Waleed

30 July 2024 1,061 5 View

Could you try using PeptiCloud and see if it's a useful tool for biology research?

PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...

28 July 2024 4,762 2 View

Should the amount of DNA input used for ChIP-seq library preparation be matched between the control and experimental groups?

Hi all. As a beginner in ChIP-seq experiments, I hope you understand that the following questions might be somewhat basic. I am planning to perform ChIP-seq or MeDIP-seq analysis to investigate...

28 July 2024 6,938 1 View

Why binding energy is in positive number after performing mmpbsa?

I performed mmpbsa of protein-protein complex simulation on gromacs. My binding free energy is +166781. This complex is stable when experimentally produced in wet lab. In index.ndx I grouped...

19 July 2024 4,120 5 View

Printing residues in an object in Pymol?

Hi! I am currently working on a protein where I have selected residues within 10A of its bound ligand in pymol. However, I can not find a way to print out what residues are contained in that group...

15 July 2024 1,727 1 View

Looking for a bioinformatics expert especially one who has expertise in Nanoparticles docking ?

Silver Nanoparticles docking with alpha amylase and alpha glusidase .

11 July 2024 6,883 2 View

How to reduce the material coming out of the torque rheometer?

A physical mix was in vitro evaluated on a torque rheometer. The composition was predominantly recycled polypropylene (PP), yet the melt flow index (MFI) was found to be excessively high....

25 June 2024 10,154 0 View

How to design primers and run PCR in SerialCloner?

I am working on a paper for my bioinformatics class, where we are running an analysis of transcriptomics on A. thaliana col-0 about the difference in gene expression in the temperatures of 21 and...

12 June 2024 8,822 1 View

How to perform the anaysis of taxonomic on raw reads from the mixed sample?

Hi, I am very new to Bioinformatics. Recently, I have the project that aims to perform taxonomic anlysis on raw reads from mixed samples taken from environment. For example, we perfromed NGS...

09 June 2024 6,537 3 View